Institute of Feed Science, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou, People's Republic of China.
Mar Biotechnol (NY). 2013 Apr;15(2):197-205. doi: 10.1007/s10126-012-9477-9. Epub 2012 Jul 27.
Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5'UTR, a 1,485-bp open reading frame, and a 194-bp 3'UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of lipid levels and lipid sources on ATGL expression in vivo were also investigated. A high-lipid diet decreased ATGL expression in fish significantly (P < 0.01). Fish in soybean oil group exhibited significantly lower ATGL expression than fish in the fish oil and beef tallow groups (P < 0.01). These data enhance our understanding of ATGL in fish lipid metabolism.
脂肪甘油三酯脂肪酶（ATGL）最近被鉴定为一种甘油三酯（TG）特异性脂肪酶。在这项研究中，我们首先从大黄鱼中获得了一个 1820bp（GenBank ID：HQ916211）的 ATGL cDNA 片段，其 5'UTR 为 141bp，开放阅读框为 1485bp，3'UTR 为 194bp。预测的鱼类 ATGL 有 494 个氨基酸（GenBank ID：ADY89608），计算分子量为 55.1kDa。ATGL 在肝脏中高度表达，在心脏、肌肉和腹部脂肪中表达程度较低。ATGL 基因表达在 4.5g 时较高，然后在 157.9g 时降低，在 474.2g 时再次升高。还研究了体内脂质水平和脂质来源对 ATGL 表达的影响。高脂饮食显著降低了鱼类 ATGL 的表达（P<0.01）。与鱼油和牛脂组相比，大豆油组的鱼类 ATGL 表达显著降低（P<0.01）。这些数据增强了我们对鱼类脂肪代谢中 ATGL 的理解。
