Guo Samuel X, Bourgeois Frederic, Chokshi Trushal, Durr Nicholas J, Hilliard Massimo A, Chronis Nikos, Ben-Yakar Adela
Department of Mechanical Engineering, The University of Texas at Austin, 204 E. Dean Keeton Street, Austin, Texas 78712, USA.
Nat Methods. 2008 Jun;5(6):531-3. doi: 10.1038/nmeth.1203. Epub 2008 Apr 13.
A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings. Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the absence of anesthetics.
要全面了解秀丽隐杆线虫中的神经再生,需要进行飞秒激光纳米轴突切断术,同时尽量减少对线虫的影响。我们展示了一种满足此类标准的微流控装置,它可以轻松实现自动化,以进行高通量基因和药理学筛选。使用“纳米轴突切断术”芯片,我们发现轴突再生的速度比之前描述的要快得多,值得注意的是,在没有麻醉剂的情况下,切断轴突的远端片段会重新生长。