Wanner L A, Gruissem W
Department of Plant Biology, University of California, Berkeley 94720.
Plant Cell. 1991 Dec;3(12):1289-303. doi: 10.1105/tpc.3.12.1289.
The tomato rbcS gene family is composed of five genes (rbcS1, 2, 3A, 3B, and 3C) that are differentially expressed during tomato development. Nuclear run-on transcription assays and RNA analysis were used to determine the contribution of transcriptional and post-transcriptional regulation to the accumulation of mRNA from the five rbcS genes in tomato seedlings, leaves, and fruit. We found that the qualitative pattern of mRNA accumulation is regulated at the transcriptional level and that, in general, there is a correlation of rates of rbcS transcription with overall rbcS mRNA abundance in fruit and leaves. Although transcriptional control is a primary determinant for rbcS gene expression in tomato, examination of relative transcription rates and mRNA accumulation of each rbcS gene demonstrated that there is also significant post-transcriptional control of rbcS gene expression during organ development. Individual rbcS mRNAs, which have highly conserved coding sequences and differ only in their 5' and 3' untranslated sequences, have different stabilities. We showed that both transcription and stability of individual rbcS mRNAs are altered in different organs and by the developmental program within these organs as well as by exposure to light. Together, the results provide a comprehensive analysis of the extent of transcriptional and post-transcriptional control that operates within the rbcS gene family during plant development.
番茄rbcS基因家族由五个基因（rbcS1、2、3A、3B和3C）组成，这些基因在番茄发育过程中差异表达。利用核转录分析和RNA分析来确定转录调控和转录后调控对番茄幼苗、叶片和果实中五个rbcS基因mRNA积累的贡献。我们发现，mRNA积累的定性模式在转录水平受到调控，并且总体而言，rbcS转录速率与果实和叶片中rbcS mRNA的总体丰度之间存在相关性。虽然转录调控是番茄中rbcS基因表达的主要决定因素，但对每个rbcS基因的相对转录速率和mRNA积累的检测表明，在器官发育过程中，rbcS基因表达也存在显著的转录后调控。具有高度保守编码序列且仅在5'和3'非翻译序列上有所不同的各个rbcS mRNA，具有不同的稳定性。我们表明，各个rbcS mRNA的转录和稳定性在不同器官中以及受这些器官内的发育程序影响，同时也受光照影响而发生改变。这些结果共同对植物发育过程中rbcS基因家族内转录调控和转录后调控的程度进行了全面分析。
