Kravchenko A N, Larionova A Ia, Miliutin L I
Genetika. 2008 Jan;44(1):45-53.
Based on the analysis of 22 loci controlling allozyme variation of MDH, SKDH, 6-PGD, IDH, PEPCA, GOT, FDH, LAP, PGI, PGM, SOD, and GDH, the data on within- and among-population variation for nine cenopopulations of Siberian spruce (Picea obovata Ledeb.) located along the Yenisei meridian, from 65 degrees 50' NL to 52degrees 14' NL, were obtained. It was demonstrated that 86.36% of the loci, tested in the species analyzed, were polymorphic. The mean number of alleles per locus was 2.91, and the observed and expected heterozygosity constituted 0.161 and 0.168, respectively. More than 97% of total variation occurred within the populations, while the proportion of the among-population variation constituted only 2.3% (Fst =0.0230). Genetic distance (DN) between the populations examined varied from 0.0019 to 0.0115, averaging at 0.0051. It was shown that in the part of the Siberian spruce range examined there was no close association between the level of the genetic differentiation of the populations and the geographic distance between them. It seems likely, that this finding can be associated with the fact that Siberian spruce, growing on this territory, is the intrazonal species, i.e., it is not attached to a certain forest zone, and its distribution is mostly determined by local ecological conditions.
基于对控制苹果酸脱氢酶（MDH）、琥珀酸脱氢酶（SKDH）、6 - 磷酸葡萄糖脱氢酶（6 - PGD）、异柠檬酸脱氢酶（IDH）、磷酸烯醇丙酮酸羧化酶（PEPCA）、谷草转氨酶（GOT）、甲酸脱氢酶（FDH）、亮氨酸氨肽酶（LAP）、磷酸葡萄糖异构酶（PGI）、磷酸葡萄糖变位酶（PGM）、超氧化物歧化酶（SOD）和谷氨酸脱氢酶（GDH）等位酶变异的22个位点的分析，获得了沿叶尼塞子午线从北纬65度50′到52度14′分布的9个西伯利亚云杉（Picea obovata Ledeb.）群落内和群落间变异的数据。结果表明，在所分析的物种中，86.36%的测试位点是多态的。每个位点的平均等位基因数为2.91，观察到的杂合度和预期杂合度分别为0.161和0.168。总变异的97%以上发生在种群内，而种群间变异的比例仅为2.3%（Fst = 0.0230）。所研究种群之间的遗传距离（DN）从0.0019到0.0115不等，平均为0.0051。结果表明，在所研究的西伯利亚云杉分布范围内，种群的遗传分化水平与它们之间的地理距离没有密切关联。这一发现可能与以下事实有关：生长在该地区的西伯利亚云杉是地带内物种，即它不局限于某一特定森林带，其分布主要由当地生态条件决定。
