Mel'nikova M N, Petrov N B, Lomov A A, la Porta N, Politov D V
Genetika. 2012 May;48(5):660-5.
From a clone library containing microsatellite DNA fragments of Norwegian spruce, seven pairs of primers were selected. These primers were tested to be the markers in the genetic structure analysis of nine populations of Eurasian spruce species Picea abies (L.) Karst. and Picea obovata Ledeb. Five pairs of these primers identified polymorphic loci with the allele numbers from 6 to 15. In the populations examined, the observed and expected heterozygosity values assessed at five loci varied from 0.1778 to 0.6556 and from 0.7800 to 0.900, respectively. In the populations examined, the values of F(st) index varied from 0.0691 to 0.2551 with the mean value of 0.1318. On the dendrogram based on Nei genetic distances, the populations formed three groups: Pskov-Ciscarpathia, Komi-Tatarstan-Arkhangelsk, Kazakhstan-Karelia(natural)-Karelia(culture)-Krasnoyarsk. Five of the primer pairs tested proved useful for analysis of the population genetic structure in Eurasian spruce species.
从一个包含挪威云微卫星DNA片段的克隆文库中，选出了7对引物。这些引物被用于欧洲云杉9个种群的遗传结构分析，作为标记，这9个种群包括欧洲云杉（Picea abies (L.) Karst.）和新疆云杉（Picea obovata Ledeb.）。其中5对引物鉴定出了等位基因数在6到15之间的多态性位点。在所检测的种群中，在5个位点评估的观察杂合度值和期望杂合度值分别在0.1778至0.6556和0.7800至0.900之间变化。在所检测的种群中，F(st)指数值在0.0691至0.2551之间变化，平均值为0.1318。基于Nei遗传距离的聚类图上，这些种群形成了三组：普斯科夫-喀尔巴阡地区、科米-鞑靼斯坦-阿尔汉格尔斯克、哈萨克斯坦-卡累利阿（自然）-卡累利阿（人工）-克拉斯诺亚尔斯克。所测试的5对引物被证明可用于欧亚云杉物种的种群遗传结构分析。
