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用源自不同雄兔的经氚标记胸腺嘧啶核苷和碳-14标记的精子对兔卵进行受精后的放射自显影研究。

Autoradiographic studies of rabbit ova after fertilization with thymidine-H3 and -C14 labelled spermatozoa originating from different bucks.

作者信息

Prinzen R

出版信息

Int J Fertil. 1977;22(1):36-9.

PMID:18410
Abstract

Rabbit spermatozoa were labelled in vivo injecting male rabbits with thymidine-H3 and thymidine-C14. Ova fertilized with H3-labelled spermatozoa were found to contain more silver grains when they had cleaved several times than before cleavage. Fertilized ova originating from rabbits that had been mated with both an H3- and C14-thymidine labelled buck carried both labels. An explanation for this phenomenon may be that additional spermatozoa in the perivitelline space of fertilized ova pass labelled DNA-bases to the cleaving blastomeres.

摘要

给雄兔注射氚标记的胸腺嘧啶核苷和碳-14标记的胸腺嘧啶核苷，在体内对兔精子进行标记。发现用氚标记精子受精的卵子在多次分裂后比分裂前含有更多的银颗粒。与同时注射了氚标记和碳-14标记胸腺嘧啶核苷的雄兔交配的母兔所产生的受精卵同时带有这两种标记。对这一现象的一种解释可能是，受精卵卵周隙中的额外精子将标记的DNA碱基传递给正在分裂的卵裂球。

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Int J Fertil. 1977;22(1):36-9.
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