Onishchenko G E, Filichkina N M, Chentsov Iu S
Tsitologiia. 1978;20(1):51-7.
For the identification of the position of individual cells in G1, S- and G2-periods of mitotic cycle in any heteroploid cell culture, it is suggested to use the Wimber and Quastler radioautographical method of double labeling of cells. It was shown that independent of the basal polidy of the cells by the successive impulse labeling of the cells with H3- and C14-thymidine with the time interval as long as G2 + M, the cells of the G1-period are unlabeled, S-cells are double labeled with C14- and C14 + H3, and G2-cells have only H3-label.
为了确定任何异倍体细胞培养中处于有丝分裂周期G1期、S期和G2期的单个细胞的位置，建议使用温伯和夸斯特勒的细胞双标记放射自显影法。结果表明，通过用H3-和C14-胸腺嘧啶核苷对细胞进行连续脉冲标记，时间间隔长达G2 + M期，无论细胞的基础倍性如何，G1期的细胞未被标记，S期细胞被C14-和C14 + H3双标记，G2期细胞仅具有H3标记。
