饮食中的脂质摄入会影响人体红细胞中与血红蛋白结合的胆固醇水平。

Dietary lipid intake influences the level of cholesterol bound to haemoglobin in human erythrocytes.

作者信息

Nikolic Milan, Ristic Medic Danijela, Stanic Dragana, Postic Marija, Arsic Aleksandra, Niketic Vesna

机构信息

Faculty of Chemistry, University of Belgrade, Studentski trg 16, P.O. Box 158, 11000 Belgrade, Serbia.

出版信息

Eur J Nutr. 2008 Apr;47(3):123-30. doi: 10.1007/s00394-008-0705-z. Epub 2008 Apr 14.

DOI:10.1007/s00394-008-0705-z

PMID:18414769

Abstract

BACKGROUND

Blood cholesterol levels are affected by diet and in particular by the type of fat intake. We originally showed that a significant but variable amount of cholesterol is firmly bound to haemoglobin (Hb) yielding the Hb-lipid adduct (Hb-Ch) in erythrocytes isolated from normo-lipidemic males.

AIM OF THE STUDY

To establish whether dietary lipids affect the level of Hb-Ch in human erythrocytes.

METHODS

Seventy-four healthy free-living adults were separated according to their serum cholesterol levels into two groups: normo-cholesterolemic (LDL cholesterol <3.4 mmol/l and total cholesterol <5.2 mmol/l) (NC) and hyper-cholesterolemic (LDL cholesterol >or=3.4 mmol/l) (HC). Habitual dietary information was used to classify subjects in both study groups into sub-groups of low-fat (<or=30% total energy as fat) and high-fat consumers (>30% total energy as fat). The NC low-fat consumers were placed on a high-lipid (high-fat and high-cholesterol) diet whereas the HC subjects with high-fat intake were assigned to a low-lipid (low-fat and low-cholesterol) diet. Both types of dietary intervention were allowed to continue for 6 weeks. The main variable under scrutiny was the Hb-Ch concentration.

RESULTS

In both study groups low-fat intake subjects had low levels of Hb-Ch (approx. 0.35 mmol/l RBC) compared with high-fat intake subjects (approx. 0.60 mmol/l RBC), and serum cholesterol was not correlated with Hb-Ch. The two dietary interventions produced substantial changes in the Hb-Ch level that paralleled variation in the serum cholesterol concentration. A high-lipid diet (35% fat, 15% saturated; 580 mg cholesterol) increased Hb-Ch (by approximately 47%, P<0.001) in subjects with low Hb-Ch at onset, whereas a low-lipid diet (28% fat, 9% saturated; 280 mg cholesterol) decreased Hb-Ch (by approximately 40%, P<0.001) in subjects with high Hb-Ch at onset.

CONCLUSION

High consumption of dietary lipids, including saturated fat and cholesterol, has an important influence on the level of Hb-Ch in human erythrocytes.

摘要

背景

血液胆固醇水平受饮食影响，尤其是脂肪摄入类型。我们最初发现，在从血脂正常的男性中分离出的红细胞中，有相当数量但可变的胆固醇与血红蛋白（Hb）紧密结合，形成血红蛋白 - 脂质加合物（Hb - Ch）。

研究目的

确定饮食中的脂质是否会影响人体红细胞中Hb - Ch的水平。

方法

74名健康的自由生活成年人根据其血清胆固醇水平分为两组：正常胆固醇血症组（低密度脂蛋白胆固醇<3.4 mmol/l且总胆固醇<5.2 mmol/l）（NC）和高胆固醇血症组（低密度脂蛋白胆固醇≥3.4 mmol/l）（HC）。使用习惯性饮食信息将两个研究组中的受试者分为低脂（脂肪占总能量≤30%）和高脂消费者（脂肪占总能量>30%）亚组。NC低脂消费者采用高脂（高脂肪和高胆固醇）饮食，而高脂摄入的HC受试者则采用低脂（低脂肪和低胆固醇）饮食。两种饮食干预均持续6周。主要研究变量是Hb - Ch浓度。

结果

在两个研究组中，低脂摄入受试者的Hb - Ch水平（约0.35 mmol/l红细胞）均低于高脂摄入受试者（约0.60 mmol/l红细胞），且血清胆固醇与Hb - Ch无相关性。两种饮食干预使Hb - Ch水平发生了显著变化，这与血清胆固醇浓度的变化平行。高脂饮食（35%脂肪，15%饱和脂肪；580 mg胆固醇）使起始Hb - Ch水平低的受试者的Hb - Ch升高（约47%，P<0.001），而低脂饮食（28%脂肪，9%饱和脂肪；280 mg胆固醇）使起始Hb - Ch水平高的受试者的Hb - Ch降低（约40%，P<0.001）。