正常人红细胞中与血红蛋白结合的胆固醇：循环中一种新的胆固醇形式？
Cholesterol bound to hemoglobin in normal human erythrocytes: a new form of cholesterol in circulation?
作者信息
Nikolić Milan, Stanić Dragana, Antonijević Nadezda, Niketić Vesna
机构信息
Department of Chemistry, University of Belgrade, Belgrade, SCG, Yugoslavia.
出版信息
Clin Biochem. 2004 Jan;37(1):22-6. doi: 10.1016/j.clinbiochem.2003.10.002.
OBJECTIVE
To study lipid fraction that is occasionally observed in red blood cell (RBC) hemolysate (supernatants from which membranes were separated).
STUDY DESIGN
Plasma lipid profiles, cholesterol (Ch) and phospholipids (PL) in intact RBCs, RBC membranes and hemolysates were examined in young healthy male population in winter and summer.
RESULTS
The RBC Ch and PL content was significantly higher than in membranes, both in winter and summer. The "excess" of cholesterol (associated with phospholipid) was bound to hemoglobin yielding Hb-lipid adduct (Hb-Ch), the pools in the RBC membrane remaining virtually unaltered. Levels of hemoglobin-lipid complex (Hb-Ch), which were significantly higher in winter than in summer (30% and 19% of the total Hb, respectively), positively correlated with plasma HDL cholesterol levels.
CONCLUSION
To our knowledge, this is the first demonstration of cholesterol binding to Hb. The results suggest influence of plasma lipoprotein metabolism on the formation of Hb-Ch.
目的
研究在红细胞（RBC）溶血产物（分离出膜后的上清液）中偶尔观察到的脂质组分。
研究设计
在冬季和夏季对年轻健康男性人群的血浆脂质谱、完整红细胞、红细胞膜和溶血产物中的胆固醇（Ch）和磷脂（PL）进行检测。
结果
红细胞中的Ch和PL含量在冬季和夏季均显著高于细胞膜中的含量。（与磷脂相关的）过量胆固醇与血红蛋白结合，产生血红蛋白 - 脂质加合物（Hb - Ch），红细胞膜中的含量几乎保持不变。血红蛋白 - 脂质复合物（Hb - Ch）的水平在冬季显著高于夏季（分别占总血红蛋白的30%和19%），与血浆高密度脂蛋白胆固醇水平呈正相关。
结论
据我们所知，这是胆固醇与血红蛋白结合的首次证明。结果表明血浆脂蛋白代谢对Hb - Ch形成的影响。
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