Samanta T K, Bhattacharyya R
Department of Botany, Burdwan University, Golapbag, India.
Folia Microbiol (Praha). 1991;36(1):59-66. doi: 10.1007/BF02935823.
Using mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, a number of homoserine auxotrophs have been isolated from a glutamate-producing Arthrobacter globiformis excreting L-lysine in good amounts. For further improvement, mutants resistant to the lysine analog S-(2-aminoethyl)-L-cysteine have been isolated from homoserine auxotrophs. For the three potent mutants tested, White's medium was found to be the best. Glucose, ammonium nitrate and biotin were found to be optimum at 280 mmol/L, 40 mmol/L and 22 nmol/L, respectively. With optimal glucose, ammonium nitrate and biotin, the strain AECrVI yielded 36 g lysine per L in flask culture.
利用N-甲基-N'-硝基-N-亚硝基胍诱变，从大量分泌L-赖氨酸的产谷氨酸球形节杆菌中分离出了许多高丝氨酸营养缺陷型菌株。为了进一步改良，又从高丝氨酸营养缺陷型菌株中分离出了对赖氨酸类似物S-(2-氨基乙基)-L-半胱氨酸具有抗性的突变体。对于所测试的三个高效突变体，发现怀特培养基是最佳的。葡萄糖、硝酸铵和生物素的最佳浓度分别为280 mmol/L、40 mmol/L和22 nmol/L。在最佳的葡萄糖、硝酸铵和生物素条件下，菌株AECrVI在摇瓶培养中每升可产生36克赖氨酸。
