水痘-带状疱疹病毒野生型和疫苗株中9种糖蛋白基因的比较分析。

Comparative analyses of the 9 glycoprotein genes found in wild-type and vaccine strains of varicella-zoster virus.

作者信息

Storlie Johnathan, Maresova Lucie, Jackson Wallen, Grose Charles

机构信息

Departments of Pediatrics and Microbiology, University of Iowa, Iowa City, IA, USA.

出版信息

J Infect Dis. 2008 Mar 1;197 Suppl 2:S49-53. doi: 10.1086/522127.

DOI:10.1086/522127

PMID:18419408

Abstract

The complete DNA sequences of wild-type and vaccine strains of varicella-zoster virus have been published and listed in GenBank. In this comparative genomic analysis, the sequences of the 9 glycoprotein open reading frames (ORFs) were compared. They included gE (ORF68), gI (ORF 67), gC (ORF14), gH (ORF37), gL (ORF60), gB (ORF31), gK (ORF5), gM (ORF50), and gN (ORF8 or ORF9A). After realignment on the basis of newer data, the corrected gB sequence was lengthened to include 931 residues. The data showed that there were glycoprotein polymorphisms that differentiated North American/European strains from Japanese strains-for example, an additional ATG codon in the gL of all Oka strains. Also, there were a small number of coding single-nucleotide polymorphisms present only in glycoproteins of vaccine strains. Because these changes were highly conserved, the structure of the glycoprotein was unlikely to be altered.

摘要

水痘带状疱疹病毒野生型和疫苗株的完整DNA序列已发表并列入GenBank。在本次比较基因组分析中，对9个糖蛋白开放阅读框（ORF）的序列进行了比较。它们包括gE（ORF68）、gI（ORF 67）、gC（ORF14）、gH（ORF37）、gL（ORF60）、gB（ORF31）、gK（ORF5）、gM（ORF50）和gN（ORF8或ORF9A）。根据更新的数据重新比对后，校正后的gB序列延长至包含931个残基。数据表明，存在糖蛋白多态性，可区分北美/欧洲毒株与日本毒株——例如，所有Oka株的gL中都有一个额外的ATG密码子。此外，仅在疫苗株的糖蛋白中存在少量编码单核苷酸多态性。由于这些变化高度保守，糖蛋白的结构不太可能改变。