单纯疱疹病毒1型DNA的退火与杂交特性
Annealing and hybridization properties of herpes simplex virus type 1 DNA.
作者信息
Cedar H
出版信息
J Gen Virol. 1976 Sep;32(3):337-47. doi: 10.1099/0022-1317-32-3-337.
The hybridization properties of the herpes simplex virus type 1 (HSV) genome have been analysed. The DNA has a kinetic complexity of 1 X 10(-8). E. cole RNA polymerase was found to initiate synthesis at about 70 sites on the HSV DNA. The in vitro RNA product from this reaction was complementary to about 80% of the HSV genome. The RNA-DNA hybridization rate constant (Kh) was determined using conditions of both RNA excess and DNA excess. Using this rate constant one can analyse the content of HSV sequences in any RNA population.
对单纯疱疹病毒1型（HSV）基因组的杂交特性进行了分析。该DNA的动力学复杂度为1×10⁻⁸。发现大肠杆菌RNA聚合酶在HSV DNA上约70个位点起始合成。此反应的体外RNA产物与约80%的HSV基因组互补。在RNA过量和DNA过量的条件下测定了RNA-DNA杂交速率常数（Kh）。利用该速率常数可以分析任何RNA群体中HSV序列的含量。
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