Locker H, Frenkel N
IARC Sci Publ (1971). 1978(24 Pt 1):75-85.
High-density DNA prepared from serially passaged herpes simplex virus contains three major classes of modified viral DNA molecules. The altered DNA molecules are composed of multiple repetitions of sequences derived from the right-hand side of the S region of the parental plaque-purified viral DNA. The repeat units contained in the three types of high-density DNA share most of their DNA sequences but differ with respect to a small region derived from the unique sequences of the S component of HSV-1 DNA. Hybridization of the defective DNA to HSV-infected cell RNA shows that the high-density DNA contains sequences complementary to both early and late viral transcripts.
从连续传代的单纯疱疹病毒制备的高密度DNA含有三类主要的修饰病毒DNA分子。这些改变的DNA分子由来自亲代噬斑纯化病毒DNA的S区域右侧的序列的多个重复组成。三种类型的高密度DNA中包含的重复单元共享其大部分DNA序列，但在源自HSV-1 DNA的S组分的独特序列的一个小区域方面有所不同。缺陷DNA与HSV感染细胞RNA的杂交表明，高密度DNA包含与早期和晚期病毒转录本互补的序列。
