感染非洲猪瘟病毒的细胞中DNA的合成。
Synthesis of DNA in cells infected with African swine fever virus.
作者信息
Tabares E, Sánchez Botija C
出版信息
Arch Virol. 1979;61(1-2):49-59. doi: 10.1007/BF01320591.
PMID:117788
Abstract
Incorporation of 14C-thymidine by cells infected with African swine fever virus (ASFV) occurs in the nucleus. Part of this DNA is transferred to the cytoplasm and becomes resistant to DNAse. The nuclear fraction washed with Triton X100 retained all labeled DNA and was able to synthesize viral and cellular DNA under in vitro conditions in the presence of the four deoxynucleoside triphosphates, Mg+2, and sucrose. Under similar conditions nuclei from uninfected cells synthesized very little DNA.
摘要
感染非洲猪瘟病毒（ASFV）的细胞对14C-胸苷的摄取发生在细胞核中。部分这种DNA会转移到细胞质中，并变得对脱氧核糖核酸酶有抗性。用曲拉通X100洗涤后的细胞核部分保留了所有标记的DNA，并且在四种脱氧核苷三磷酸、Mg+2和蔗糖存在的体外条件下能够合成病毒和细胞DNA。在类似条件下，未感染细胞的细胞核合成的DNA很少。
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