单纯疱疹病毒1型感染的KB细胞核中合成的RNA的特性分析。
Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.
作者信息
Miovic M L, Pizer L I
出版信息
J Virol. 1980 Jan;33(1):567-71. doi: 10.1128/JVI.33.1.567-571.1980.
Abstract
Nuclei isolated from herpes simplex virus (HSV)-infected KB cells actively synthesize HSV RNA in vitro; the RNA can be hybridized with HSV DNA or nuclear RNA from HSV-infected cells. Nascent RNA molecules labeled in vivo with 32PO4 were elongated, utilizing the nuclear system to incorporate Hg-CTP at their 3' ends, and then isolated on an affinity column. Hybridization of isolated nascent RNA molecules showed that greater than 50% of them were HSV specific and that more than 25% were self-complementary.
摘要
从单纯疱疹病毒（HSV）感染的KB细胞中分离出的细胞核在体外能积极合成HSV RNA；该RNA可与HSV DNA或来自HSV感染细胞的核RNA杂交。用32PO4在体内标记的新生RNA分子被延长，利用核系统在其3'端掺入Hg-CTP，然后在亲和柱上分离。对分离出的新生RNA分子进行杂交显示，其中超过50%是HSV特异性的，超过25%是自我互补的。
相似文献
1
Characterization of RNA synthesized in isolated nuclei of herpes simplex virus type 1-infected KB cells.单纯疱疹病毒1型感染的KB细胞核中合成的RNA的特性分析。
J Virol. 1980 Jan;33(1):567-71. doi: 10.1128/JVI.33.1.567-571.1980.
2
RNA synthesis in cells infected with herpes simplex virus. VII. Control of transcription and of transcript abundancies of unique and common sequences of herpes simplex virus 1 and 2.感染单纯疱疹病毒的细胞中的RNA合成。VII. 单纯疱疹病毒1型和2型独特及共同序列转录及转录本丰度的调控
J Virol. 1973 Jun;11(6):886-92. doi: 10.1128/JVI.11.6.886-892.1973.
3
Transcription of herpes simplex type 1 DNA in nuclei isolated from infected HEp-2 and KB cells.
Virology. 1974 Jul;60(1):302-7. doi: 10.1016/0042-6822(74)90390-0.
4
Nucleic acid biosynthesis in nuclei of cell infected with herpesviruses (HSV and EBV).感染疱疹病毒（单纯疱疹病毒和爱泼斯坦-巴尔病毒）的细胞细胞核中的核酸生物合成。
IARC Sci Publ (1971). 1975(11 Pt 1):245-57.
5
Metabolic stability of nuclear self-complementary RNA in cells infected with herpes simplex virus.单纯疱疹病毒感染细胞中核内自我互补RNA的代谢稳定性
Biochem Biophys Res Commun. 1978 Aug 14;83(3):800-5. doi: 10.1016/0006-291x(78)91465-1.
6
Synthesis of herpes simplex virus type 1 (HSV-1) DNA in isolated nuclei. II. Covalent linkage of RNA to nascent viral DNA.单纯疱疹病毒1型（HSV - 1）DNA在分离细胞核中的合成。II. RNA与新生病毒DNA的共价连接。
Intervirology. 1974;4(1):14-22. doi: 10.1159/000149839.
7
Synthesis of herpes simplex virus-specified RNA by an RNA polymerase II in isolated nuclei in vitro.单纯疱疹病毒特异性RNA在体外分离细胞核中由RNA聚合酶II合成。
Virology. 1976 May;71(1):302-11. doi: 10.1016/0042-6822(76)90114-8.
8
Annealing and hybridization properties of herpes simplex virus type 1 DNA.单纯疱疹病毒1型DNA的退火与杂交特性
J Gen Virol. 1976 Sep;32(3):337-47. doi: 10.1099/0022-1317-32-3-337.
9
Quantitation of herpes simplex virus type 1 RNA in infected HeLa cells.感染的HeLa细胞中单纯疱疹病毒1型RNA的定量分析。
J Virol. 1977 Mar;21(3):889-901. doi: 10.1128/JVI.21.3.889-901.1977.
10
Synthesis of herpes simplex virus, vaccinia virus, and adenovirus DNA in isolated HeLa cell nuclei. I. Effect of viral-specific antisera and phosphonoacetic acid.单纯疱疹病毒、痘苗病毒和腺病毒DNA在离体HeLa细胞核中的合成。I. 病毒特异性抗血清和膦甲酸的作用。
J Virol. 1975 Dec;16(6):1584-92. doi: 10.1128/JVI.16.6.1584-1592.1975.
引用本文的文献
1
Functional genomic analysis of herpes simplex virus type 1 counteraction of the host innate response.单纯疱疹病毒1型对宿主固有免疫反应的对抗作用的功能基因组分析
J Virol. 2006 Aug;80(15):7600-12. doi: 10.1128/JVI.00333-06.
2
Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.内源性核酸内切酶诱导的DNA片段化：细胞介导的细胞溶解中的早期事件。
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6361-5. doi: 10.1073/pnas.80.20.6361.
3
Herpes simplex virus-induced changes in cellular and adenovirus RNA metabolism in an adenovirus type 5-transformed human cell line.单纯疱疹病毒对5型腺病毒转化的人细胞系中细胞和腺病毒RNA代谢的影响
J Virol. 1982 May;42(2):474-87. doi: 10.1128/JVI.42.2.474-487.1982.
4
Brain-reactive autoantibody levels in the sera of ageing autoimmune mice.衰老自身免疫小鼠血清中的脑反应性自身抗体水平
Clin Exp Immunol. 1987 Oct;70(1):74-83.
5
Polyomavirus DNA is damaged in target cells during cytotoxic T-lymphocyte-mediated killing.在细胞毒性T淋巴细胞介导的杀伤过程中，多瘤病毒DNA在靶细胞中受损。
J Virol. 1989 Feb;63(2):572-8. doi: 10.1128/JVI.63.2.572-578.1989.
6
Immunogenic DNA-related factors. Nucleosomes spontaneously released from normal murine lymphoid cells stimulate proliferation and immunoglobulin synthesis of normal mouse lymphocytes.免疫原性DNA相关因子。从正常小鼠淋巴细胞中自发释放的核小体可刺激正常小鼠淋巴细胞的增殖和免疫球蛋白合成。
J Clin Invest. 1990 May;85(5):1487-96. doi: 10.1172/JCI114595.
本文引用的文献
1
State of adenovirus 2 deoxyribonucleic acid in the nucleus and its mode of transcription: studies with isolated viral deoxyribonucleic acid-protein complexes and isolated nuclei.腺病毒2型脱氧核糖核酸在细胞核中的状态及其转录模式：对分离的病毒脱氧核糖核酸-蛋白质复合物和分离细胞核的研究
J Virol. 1972 Apr;9(4):627-35. doi: 10.1128/JVI.9.4.627-635.1972.
2
Transcription of the adenovirus genome by an -amanitine-sensitive ribonucleic acid polymerase in HeLa cells.腺病毒基因组在HeLa细胞中由一种对α-鹅膏蕈碱敏感的核糖核酸聚合酶进行转录。
J Virol. 1972 Apr;9(4):621-6. doi: 10.1128/JVI.9.4.621-626.1972.
3
Specific inhibition of nuclear RNA polymerase II by alpha-amanitin.α-鹅膏蕈碱对核RNA聚合酶II的特异性抑制作用。
Science. 1970 Oct 23;170(3956):447-9. doi: 10.1126/science.170.3956.447.
4
Role of DNA-dependent RNA polymerases II and III in transcription of the adenovirus genome late in productive infection.DNA依赖性RNA聚合酶II和III在腺病毒基因组生产性感染后期转录中的作用。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3426-39. doi: 10.1073/pnas.71.9.3426.
5
Transcription of herpes simplex type 1 DNA in nuclei isolated from infected HEp-2 and KB cells.
Virology. 1974 Jul;60(1):302-7. doi: 10.1016/0042-6822(74)90390-0.
6
Deoxyribonucleic acid synthesis in synchronized mammalian KB cells infected with herpes simplex virus.感染单纯疱疹病毒的同步化哺乳动物KB细胞中的脱氧核糖核酸合成
J Virol. 1971 Jun;7(6):783-91. doi: 10.1128/JVI.7.6.783-791.1971.
7
Viral RNA synthesis and levels of DNA-dependent RNA polymerases during replication of adenovirus 2.腺病毒2型复制过程中的病毒RNA合成及依赖DNA的RNA聚合酶水平
J Virol. 1975 Jan;17(1):114-26. doi: 10.1128/JVI.17.1.114-126.1976.
8
RNA aggregation during sulfhydryl-agarose chromatography of mercurated RNA.汞化RNA在巯基琼脂糖色谱法中的RNA聚集
Nucleic Acids Res. 1977 Jun;4(6):1979-88. doi: 10.1093/nar/4.6.1979.
9
Fidelity of adenovirus RNA transcription in isolated HeLa cell nuclei.腺病毒RNA转录在分离的HeLa细胞核中的保真度。
J Virol. 1977 May;22(2):290-9. doi: 10.1128/JVI.22.2.290-299.1977.
10
Use of mercury-substituted ribonucleoside triphosphates can lead to artefacts in the analysis of in vitro chromatin transcrits.使用汞取代的核糖核苷三磷酸可能会在体外染色质转录物分析中导致假象。
Biochem Biophys Res Commun. 1977 Apr 11;75(3):598-603. doi: 10.1016/0006-291x(77)91514-5.
Zotero 插件