Tang Cha-Min
Baltimore VA Medical Center, University of Maryland School of Medicine, Baltimore, Maryland, USA.
Curr Protoc Neurosci. 2006 Nov;Chapter 6:Unit 6.21. doi: 10.1002/0471142301.ns0621s37.
Photolysis of "caged" compounds is a technique for releasing biologically active compounds in which the timing, rate, and spatial profile of release are controlled by light. Issues relating to the delivery of light for single-photon photolysis are presented. Specific discussions include the theories relating to how light interacts with biological tissue to produce scattering and phototoxicity, as well as the issues involved in choosing the appropriate light source. Several approaches and optical designs are presented for delivering the output of a laser to a microscopic specimen. The criteria for choosing an approach are presented. The commercial sources for the parts needed to build a photolysis system are also provided. This unit will be particularly useful for investigators interested in single-photon photolysis of caged neurotransmitters in brain slices.
“笼形”化合物的光解是一种释放生物活性化合物的技术，其中释放的时间、速率和空间分布由光控制。文中介绍了与单光子光解的光传输相关的问题。具体讨论包括光与生物组织相互作用产生散射和光毒性的相关理论，以及选择合适光源所涉及的问题。文中介绍了几种将激光输出传输到微观标本的方法和光学设计。还介绍了选择方法的标准。此外，还提供了构建光解系统所需部件的商业来源。本单元对于对脑片笼形神经递质的单光子光解感兴趣的研究人员将特别有用。
