Seidman C E, Struhl K
Harvard Medical School, Boston, Massachusetts, USA.
Curr Protoc Protein Sci. 2001 May;Appendix 4:4D. doi: 10.1002/0471140864.psa04ds13.
This appendix presents a procedure for transformation using calcium chloride, and also an alternate procedure for one-step preparation and transformation of competent cells.
本附录介绍了使用氯化钙进行转化的方法,以及一种用于感受态细胞一步制备和转化的替代方法。