[诱导质粒转化效率提高的大肠杆菌K-12突变体]
[Induction of Escherichia coli K-12 mutants with an increased efficiency of plasmid transformation].
作者信息
Likhacheva N A, Molchanova E S, Il'iashenko B N
出版信息
Biull Eksp Biol Med. 1982 May;93(5):81-2.
PMID:7046841
Abstract
A new rapid method for plasmid transformation of Escherichia coli K-12 cells has been devised. It consists in application of plasmid pMB9 DNA to the surface of an agar medium with 0.05 M CaCl2 and tetracycline (50 micrograms/ml). The recipient cells treated with nitrosoguanidine were drifted on by sectors on the plates with pMB9 DNA. The method enabled the obtaining of 12 mutants with high efficiency of plasmid transformation.
摘要
已设计出一种用于大肠杆菌K-12细胞质粒转化的新的快速方法。该方法是将质粒pMB9 DNA应用于含有0.05 M氯化钙和四环素（50微克/毫升）的琼脂培养基表面。用亚硝基胍处理过的受体细胞在含有pMB9 DNA的平板上被扇形接种。该方法能够高效获得12个质粒转化突变体。
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