Phelan Katy, May Kristin M
Cytogenetics Laboratory, Florida Cancer Specialists & Research Institute, Fort Myers, Florida.
Genetic Diagnostic Laboratory, Children's Hospital at Erlanger, Chattanooga, Tennessee.
Curr Protoc Hum Genet. 2017 Jul 11;94:A.3G.1-A.3G.22. doi: 10.1002/cphg.41.
Cultured mammalian cells are used extensively in the field of human genetics. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2017 by John Wiley & Sons, Inc.
培养的哺乳动物细胞在人类遗传学领域被广泛使用。为了能够在培养中保存细胞的结构、功能、行为和生物学特性，需要一些特殊技能。本单元描述了维持和保存细胞培养物所需的基本技能：保持无菌技术、制备具有适当特性的培养基、传代、冷冻和储存、复苏冷冻的细胞株以及计数活细胞。© 2017 约翰威立国际出版公司。
