Tong Xiaodong, Trivedi Archana, Jia Hongfei, Zhang Minjuan, Wang Ping
Biotechnology Institute and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108, USA.
Biotechnol Prog. 2008 May-Jun;24(3):714-9. doi: 10.1021/bp0704135. Epub 2008 Apr 23.
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 microg/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.
生物活性材料已被广泛应用于包括生物催化剂、生物传感器、防污膜及其他功能和智能材料等众多领域。我们在此报告一种通过旋涂工艺制备生物活性材料的独特方法。具体而言，我们研究了用枯草杆菌蛋白酶卡尔伯格亚种包被塑料薄膜的制备方法，并检测了它们对水解鸡蛋白（CEA）的活性。该工艺产生了典型负载量为13微克/平方厘米的酶涂层，在水溶液中保留了46%的水解CEA的酶活性。有趣的是，表面包被蛋白酶的薄膜不仅能催化水溶液中CEA的水解，而且对涂覆在酶薄膜顶部的固态CEA也表现出良好的活性。
