Hirano N, Fujiwara K, Matumoto M
Jpn J Microbiol. 1976 Jun;20(3):219-25.
Various factor sinfluencing the plaque formation of mouse hepatitis virus (MHV-2) in DBT cell monolayers were studied and a practical method for plaque assay was developed. Infected DBT cells yielded high-titered virus and were a satisfactory source of complement-fixing viral antigen. The predominant cytopathic effect of MHV-2 in DBT cells was cell rounding and detachment, but no syncytial formation was observed. Fluorescent antibody staining revealed specific fluorescence only in the cytoplasm of infected DBT cells. In one-step growth experiment, newly formed virus was first recognized within 4-hr postinfection and showed subsequently a rapid exponential increase. Release of newly formed virus from the cell was rapid, and a continuous release lasted for a certain period of time. The average per-cell yield of active virus was estimated to be about 6-7 X 10(2) plaque-forming units.
研究了影响小鼠肝炎病毒（MHV - 2）在DBT细胞单层中形成蚀斑的各种因素，并开发了一种蚀斑测定的实用方法。感染的DBT细胞产生高滴度病毒，是补体结合病毒抗原的良好来源。MHV - 2在DBT细胞中的主要细胞病变效应是细胞变圆和脱落，但未观察到多核巨细胞形成。荧光抗体染色显示仅在感染的DBT细胞的细胞质中出现特异性荧光。在一步生长实验中，新形成的病毒在感染后4小时内首次被识别，随后呈快速指数增长。新形成的病毒从细胞中释放迅速，且持续释放一段时间。活性病毒的平均每细胞产量估计约为6 - 7×10²蚀斑形成单位。
