David-West T S
Acta Microbiol Acad Sci Hung. 1975;22(1):33-40.
Dugbe virus, a new tick-borne arbovirus from Nigeria, was propagated in continuous porcine kidney (PS) cells, and the cytopathology studied by various staining techniques such as May-Grunwald-Giemsa, methyl-green pyronine, Feulgen, and immuno-fluorescence. The gross cytopathology was slight and infected cells continued to undergo normal mitotic divisions. The outstanding cytopathologic feature was the presence of large basophilic intracytoplasmic inclusion bodies, which were pyroninophilic and Feulgen negative. By immunofluorescence these inclusions were shown to be depots of viral antigen; specific fluorescence was confined to the cytoplasm throughout the replication of the virus.
杜格贝病毒是一种来自尼日利亚的新型蜱传虫媒病毒，在连续传代的猪肾（PS）细胞中繁殖，并采用多种染色技术（如May-Grunwald-Giemsa染色、甲基绿派洛宁染色、Feulgen染色和免疫荧光染色）研究其细胞病理学。大体细胞病理学变化轻微，受感染细胞继续进行正常的有丝分裂。突出的细胞病理学特征是存在大的嗜碱性胞质内包涵体，这些包涵体嗜派洛宁且Feulgen染色阴性。通过免疫荧光显示，这些包涵体是病毒抗原的储存库；在病毒整个复制过程中，特异性荧光局限于细胞质中。
