爱泼斯坦-巴尔病毒（EBV）永生化B细胞系的产生。
Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines.
作者信息
Tosato Giovanna, Cohen Jeffrey I
机构信息
National Cancer Institute, Bethesda, Maryland.
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
出版信息
Curr Protoc Immunol. 2007 Feb;Chapter 7:7.22.1-7.22.4. doi: 10.1002/0471142735.im0722s76.
PMID:18432996
Abstract
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.
摘要
EBV使B淋巴细胞永生化是诱导某些人B淋巴细胞长期生长的有效方法。本单元中描述的完成此操作的方案可分为三个阶段：病毒制备、待永生化靶细胞的制备以及EBV感染和感染细胞的生长。
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