一种快速建立人B淋巴细胞爱泼斯坦-巴尔病毒永生化的有效方法。
An efficient method for the rapid establishment of Epstein-Barr virus immortalization of human B lymphocytes.
作者信息
Oh H-M, Oh J-M, Choi S-C, Kim S-W, Han W-C, Kim T-H, Park D-S, Jun C-D
机构信息
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Chonbuk, Korea.
出版信息
Cell Prolif. 2003 Aug;36(4):191-7. doi: 10.1046/j.1365-2184.2003.00276.x.
Abstract
Several methods have been developed for the immortalization of B lymphocytes by Epstein-Barr virus (EBV). We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester-induced EBV stock significantly improved immortalization efficiency and reduced the time between initiation and immortalization and cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in immune and autistic disorders.
摘要
已经开发出几种通过爱泼斯坦-巴尔病毒（EBV）使B淋巴细胞永生化的方法。我们开发了一种高效方法，可缩短从培养开始到永生化和冷冻保存的时间。对淋巴细胞进行两次EBV感染，并使用佛波酯诱导的EBV储备液，显著提高了永生化效率，并缩短了从开始到永生化及冷冻保存之间的时间。所得细胞库用于生产DNA，以进行针对免疫和自闭症相关疾病基因的遗传学研究。
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