转录起始位点的定位对于枯草芽孢杆菌响应氨基酸饥饿时基因表达的变化至关重要。

The identity of the transcription +1 position is crucial for changes in gene expression in response to amino acid starvation in Bacillus subtilis.

作者信息

Krásný Libor, Tiserová Hana, Jonák Jirí, Rejman Dominik, Sanderová Hana

机构信息

Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology ASCR, Vídenská 1083, Prague 142 20, Czech Republic.

出版信息

Mol Microbiol. 2008 Jul;69(1):42-54. doi: 10.1111/j.1365-2958.2008.06256.x. Epub 2008 Apr 21.

DOI:10.1111/j.1365-2958.2008.06256.x

PMID:18433449

Abstract

We identify here a pattern in the transcription start sites (+1A or +1G) of sigma(A)-dependent promoters of genes that are up-/downregulated in response to amino acid starvation (stringent response) in Bacillus subtilis. Upregulated promoters initiate mostly with ATP and downregulated promoters with GTP. These promoters appear to be sensitive to changes in initiating nucleoside triphosphate concentrations. During the stringent response in B. subtilis, when ATP and GTP levels change reciprocally, the identity of the +1 position (A or G) of these promoters is a factor important in their regulation. Mutations that change the identity of position +1 (A for G and vice versa) change the response of the promoter to amino acid starvation.

摘要

我们在此确定了枯草芽孢杆菌中因氨基酸饥饿（严谨反应）而上调/下调的基因的σ(A) 依赖性启动子转录起始位点（+1A 或 +1G）的一种模式。上调的启动子大多以 ATP 起始，而下调的启动子以 GTP 起始。这些启动子似乎对起始核苷三磷酸浓度的变化敏感。在枯草芽孢杆菌的严谨反应期间，当 ATP 和 GTP 水平相互变化时，这些启动子 +1 位点（A 或 G）的身份是其调控中的一个重要因素。改变 +1 位点身份（A 变为 G 或反之亦然）的突变会改变启动子对氨基酸饥饿的反应。