Mathews H M, Moss D M, Visvesvara G S
J Protozool. 1986 Aug;33(3):328-34. doi: 10.1111/j.1550-7408.1986.tb05619.x.
Axenic cultures of Entamoeba histolytica strains HK-9, HM-1, and Rahman were fractionated to provide plasma membranes, internal, vesiculated membranes, and a soluble cytosol. Each particulate fraction was solubilized and all fractions were analyzed by techniques designed to demonstrate molecular complexity and serologic reactivity. The cytosol contained more antigenic moieties than either membrane fraction; however, the antigens associated with the membranes had very high reactivity and lower nonspecific activity than the cytosol.
对溶组织内阿米巴菌株HK - 9、HM - 1和拉赫曼的无菌培养物进行分级分离，以获得质膜、内部的泡状膜和可溶性胞质溶胶。每个颗粒级分都进行了增溶处理，所有级分都通过旨在展示分子复杂性和血清学反应性的技术进行了分析。胞质溶胶所含的抗原部分比任何一个膜级分都多；然而，与膜相关的抗原具有非常高的反应性，且非特异性活性比胞质溶胶低。
