Joyce M P, Ravdin J I
Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville.
Am J Trop Med Hyg. 1988 Jan;38(1):74-80. doi: 10.4269/ajtmh.1988.38.74.
Immune sera from 11 patients cured of amebic liver abscess was used to identify antigens of Entamoeba histolytica. Strain HM1-IMSS, among the most virulent in axenic culture, was used. The 37 and 90 Kd antigens were surface glycoproteins as indicated by lactoperoxidase iodination and by Concanavalin A blotting; the 59 Kd antigen was a mannose containing glycoprotein that did not appear to be on the cell surface. Western blots of 11 different immune sera revealed specific binding of immune IgG to 9 amebic proteins. Most frequently recognized proteins were of molecular weight 37, 59, and 90 Kd. The immunoblot pattern in 5 patients was unchanged for up to 30 months post-treatment for liver abscess.
用11例已治愈阿米巴肝脓肿患者的免疫血清来鉴定溶组织内阿米巴的抗原。使用了在无细菌培养中最具毒力的HM1-IMSS菌株。通过乳过氧化物酶碘化和伴刀豆球蛋白A印迹法表明，37和90千道尔顿抗原是表面糖蛋白；59千道尔顿抗原是一种含甘露糖的糖蛋白，似乎不在细胞表面。11种不同免疫血清的蛋白质印迹显示免疫IgG与9种阿米巴蛋白有特异性结合。最常识别的蛋白分子量为37、59和90千道尔顿。5例患者肝脓肿治疗后长达30个月，免疫印迹模式未改变。
