Ditrich O, Vokurková N, Giboda M, Kopácek P
Institute of Parasitology, Czechoslovak Academy of Sciences, Ceské Budĕjovice.
Folia Parasitol (Praha). 1988;35(4):309-15.
Soluble antigens of ten strains of E. histolytica were studied by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked electroimmunotransfer blots (EITB). No relations of immune replicas to virulence, geographical origin and method of cultivation (xenic or axenic culture) were found. Antigens of all ten strains tested precipitated with anti-E. histolytica human serum in the area of 30-43 kD. Antigen of HK-9 strain created in this area a characteristic pattern with all sera containing the specific anti-E. histolytica antibodies and, therefore, EITB can be used for excluding false positive results in ELISA.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和酶联免疫电转移印迹法（EITB）对10株溶组织内阿米巴的可溶性抗原进行了研究。未发现免疫印迹与毒力、地理来源及培养方法（单栖培养或无菌培养）之间存在关联。所检测的全部10株菌株的抗原在30-43kD区域与抗溶组织内阿米巴人血清发生沉淀反应。HK-9菌株的抗原在此区域与所有含有特异性抗溶组织内阿米巴抗体的血清产生特征性条带，因此，EITB可用于排除ELISA中的假阳性结果。
