酵母Pgc1p（YPL206c）通过磷脂酶C型降解机制控制磷脂酰甘油的含量。
Yeast Pgc1p (YPL206c) controls the amount of phosphatidylglycerol via a phospholipase C-type degradation mechanism.
作者信息
Simocková Mária, Holic Roman, Tahotná Dana, Patton-Vogt Jana, Griac Peter
机构信息
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Moyzesova 61, Ivanka pri Dunaji, 900 28, Slovakia.
出版信息
J Biol Chem. 2008 Jun 20;283(25):17107-15. doi: 10.1074/jbc.M800868200. Epub 2008 Apr 23.
Abstract
The product of the open reading frame YPL206c, Pgc1p, of the yeast Saccharomyces cerevisiae displays homology to bacterial and mammalian glycerophosphodiester phosphodiesterases. Deletion of PGC1 causes an accumulation of the anionic phospholipid, phosphatidylglycerol (PG), especially under conditions of inositol limitation. This PG accumulation was not caused by increased production of phosphatidyl-glycerol phosphate or by decreased consumption of PG in the formation of cardiolipin, the end product of the pathway. PG accumulation in the pgc1Delta strain was caused rather by inactivation of the PG degradation pathway. Our data demonstrate an existence of a novel regulatory mechanism in the cardiolipin biosynthetic pathway in which Pgc1p is required for the removal of excess PG via a phospholipase C-type degradation mechanism.
摘要
酿酒酵母开放阅读框YPL206c的产物Pgc1p与细菌和哺乳动物甘油磷酸二酯磷酸二酯酶具有同源性。PGC1的缺失会导致阴离子磷脂磷脂酰甘油（PG）的积累，特别是在肌醇限制条件下。这种PG积累不是由磷脂酰甘油磷酸产量增加或在该途径的终产物心磷脂形成过程中PG消耗减少引起的。pgc1Δ菌株中的PG积累而是由PG降解途径的失活引起的。我们的数据证明了心磷脂生物合成途径中存在一种新的调节机制，其中Pgc1p通过磷脂酶C型降解机制去除过量的PG是必需的。
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