Rossner Pavel, Svecova Vlasta, Milcova Alena, Lnenickova Zdena, Solansky Ivo, Sram Radim J
Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR vvi, Vídenská 1083, Prague 4, Czech Republic.
Mutat Res. 2008 Jul 3;642(1-2):14-20. doi: 10.1016/j.mrfmmm.2008.03.003. Epub 2008 Mar 25.
We investigated the seasonal variability of 8-oxodeoxyguanosine (8-oxodG), a marker of oxidative damage to DNA, in urine of 50 bus drivers and 50 controls in Prague, Czech Republic, in three seasons with different levels of air pollution: winter 2005, summer 2006 and winter 2006. The exposure to environmental pollutants (carcinogenic polycyclic aromatic hydrocarbons, c-PAHs, particulate matter (PM), and volatile organic compounds (VOC)) was monitored by personal and/or stationary monitors. For the analysis of 8-oxodG levels, the ELISA technique was used. Bus drivers were exposed to significantly higher levels of c-PAHs in winter 2006, while in the other two seasons the exposure of controls was unexpectedly higher than that of bus drivers. We did not see any difference in VOC exposure between both groups in summer 2006 and in winter 2006; VOC were not monitored in winter 2005. 8-OxodG levels were higher in bus drivers than in controls in all seasons. The median levels of 8-oxodG (nmol/mmol creatinine) in bus drivers vs. controls were as follows: winter 2005: 7.79 vs. 6.12 (p=0.01); summer 2006: 6.91 vs. 5.11 (p<0.01); winter 2006: 5.73 vs. 3.94 (p<0.001). Multivariate logistic regression analysis identified PM2.5 and PM10 levels, measured by stationary monitors during a 3-day period before urine collection, as the only factors significantly affecting 8-oxodG levels, while the levels of c-PAHs had no significant influence.
我们在捷克共和国布拉格对50名公交车司机和50名对照者的尿液进行了研究，以调查8-氧代脱氧鸟苷（8-oxodG，一种DNA氧化损伤标志物）在三个空气污染水平不同季节中的季节性变化：2005年冬季、2006年夏季和2006年冬季。通过个人和/或固定监测器对环境污染物（致癌多环芳烃，c-PAHs、颗粒物（PM）和挥发性有机化合物（VOC））的暴露情况进行了监测。对于8-oxodG水平的分析，采用了酶联免疫吸附测定（ELISA）技术。公交车司机在2006年冬季接触到的c-PAHs水平显著更高，而在其他两个季节，对照者的暴露水平出人意料地高于公交车司机。在2006年夏季和2006年冬季，两组之间的VOC暴露没有差异；2005年冬季未对VOC进行监测。在所有季节中，公交车司机的8-oxodG水平均高于对照者。公交车司机与对照者的8-oxodG中位数水平（nmol/mmol肌酐）如下：2005年冬季：7.79对6.12（p=0.01）；2006年夏季：6.91对5.11（p<0.01）；2006年冬季：5.73对3.94（p<0.001）。多变量逻辑回归分析确定，在尿液收集前3天通过固定监测器测量的PM2.5和PM10水平是显著影响8-oxodG水平的唯一因素，而c-PAHs水平没有显著影响。
