Rossner Pavel, Svecova Vlasta, Milcova Alena, Lnenickova Zdena, Solansky Ivo, Sram Radim J
Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR vvi, Vídenská 1083, Prague, Czech Republic.
Mutat Res. 2008 Jul 3;642(1-2):21-7. doi: 10.1016/j.mrfmmm.2008.03.004. Epub 2008 Mar 25.
The aim of the present study was to investigate the seasonal variability of markers of oxidative damage to lipids (15-F2t-isoprostane, 15-F2t-IsoP) and proteins (protein carbonyl levels) in 50 bus drivers and 50 controls from Prague, Czech Republic, and to identify factors affecting oxidative stress markers. The samples were collected in three seasons with different levels of air pollution. The exposure to environmental pollutants (carcinogenic polycyclic aromatic hydrocarbons, c-PAHs, particulate matter, PM2.5 and PM10, and volatile organic compounds, VOC) was monitored by personal and/or stationary monitors. For the analysis of both markers, ELISA techniques were used. The median levels of individual markers in bus drivers versus controls were as follows: 15-F2t-IsoP (nmol/mmol creatinine): winter 2005, 0.81 versus 0.68 (p<0.01); summer 2006, 0.62 versus 0.60 (p=0.90); winter 2006, 0.76 versus 0.51 (p<0.001); carbonyl levels (nmol/ml plasma): winter 2005, 14.1 versus 12.9 (p=0.001); summer 2006, 17.5 versus 16.6 (p=0.26); winter 2006, 13.5 versus 11.7 (p<0.001). Multivariate logistic regression identified PM levels measured by stationary monitors over a period 25-27 days before urine collection as a factor positively associated with lipid peroxidation, while protein oxidation levels correlated negatively with both c-PAHs and PM levels. In conclusion, markers of oxidative damage to lipids and proteins were increased in bus drivers in winter seasons, but not in summer. Lipid peroxidation was positively correlated with c-PAHs and PM exposure; protein oxidation correlated negatively and was highest in summer suggesting another factor(s) affecting protein carbonyl levels.
本研究的目的是调查捷克共和国布拉格的50名公交车司机和50名对照者中脂质氧化损伤标志物（15-F2t-异前列腺素，15-F2t-IsoP）和蛋白质氧化损伤标志物（蛋白质羰基水平）的季节变化，并确定影响氧化应激标志物的因素。样本在三个空气污染水平不同的季节采集。通过个人和/或固定监测器监测环境污染物（致癌多环芳烃，c-PAHs、颗粒物，PM2.5和PM10以及挥发性有机化合物，VOC）的暴露情况。对于这两种标志物的分析，采用了酶联免疫吸附测定（ELISA）技术。公交车司机与对照者中各标志物的中位数水平如下：15-F2t-IsoP（nmol/mmol肌酐）：2005年冬季，0.81对0.68（p<0.01）；2006年夏季，0.62对0.60（p=0.90）；2006年冬季，0.76对0.51（p<0.001）；羰基水平（nmol/ml血浆）：2005年冬季，14.1对12.9（p=0.001）；2006年夏季，17.5对16.6（p=0.26）；2006年冬季，13.5对11.7（p<0.001）。多变量逻辑回归分析确定，在尿液采集前25-27天期间通过固定监测器测量的PM水平是与脂质过氧化呈正相关的一个因素，而蛋白质氧化水平与c-PAHs和PM水平均呈负相关。总之，公交车司机在冬季脂质和蛋白质氧化损伤标志物增加，而夏季未增加。脂质过氧化与c-PAHs和PM暴露呈正相关；蛋白质氧化呈负相关且在夏季最高，提示存在其他影响蛋白质羰基水平的因素。
