Chosia M, Marsan C
Zakładu Patomorfologii Nowotworów Instytutu Patologii, Pomorskiej Akademii Medycznej, Szczecinie.
Pneumonol Alergol Pol. 1991;59(9-10):17-22.
51 BALFs from HIV positive patients were analysed. In 16 of them Pneumocystis carinii organisms were found. Two methods detecting Pneumocystis carinii were used: Grocott-Gomori, fluorescence Papanicolaou stain. The best staining method turned out to be Grocott-Gomori. The differential count was carried out in 40 BALFs. In patients with Pneumocystis carinii detected by BAL neutrophilia was always present. In some of these patients lymphocytosis was also present. In 4 patients with high lymphocytosis immunocytochemical examinations with monoclonal antibodies against CD4, CD8 T cells showed a marked decrease of the CD4/CD8 ratio.
对51份来自HIV阳性患者的支气管肺泡灌洗液（BALF）进行了分析。其中16份检测到卡氏肺孢子虫。采用了两种检测卡氏肺孢子虫的方法：Grocott-Gomori染色法、荧光巴氏染色法。结果表明，最佳染色方法是Grocott-Gomori染色法。对40份BALF进行了细胞分类计数。在通过BAL检测出卡氏肺孢子虫的患者中，总是存在中性粒细胞增多。在其中一些患者中也存在淋巴细胞增多。在4例淋巴细胞显著增多的患者中，使用抗CD4、CD8 T细胞的单克隆抗体进行免疫细胞化学检查显示CD4/CD8比值明显降低。
