Tyrka Miroslaw, Perovic Dragan, Wardynska Agnieszka, Ordon Frank
Laboratory of Population Genetics, Polonia University, Czestochowa, Poland.
J Appl Genet. 2008;49(2):127-34. doi: 10.1007/BF03195605.
Genomic sequence AY661558, representing a part of the BAC contig of the Rym4/Rym5 locus conferring resistance to the barley yellow mosaic virus complex (BaMMV/BaYMV), was exploited in order to develop SSR markers for practical barley breeding. Out of 57 SSR motifs found within this sequence, primers were designed and tested for the 5 SSRs with the highest repeat length. The polymorphic SSR marker QLB1 co-segregated with rym4 and rym5 phenotypes in respective high-resolution mapping populations developed for the construction of the original BAC contig. The primers targeted 2 sites located 756 bp and 5173 bp downstream of the translation initiation factor 4E (Hv-eIF4E). Physical linkage of the QLB1 marker to the Rym4/Rym5 locus was confirmed experimentally on Morex BAC 519J14, a seed BAC of Hv-eIF4E, and BAC 801A11, which is located proximally to Hv-eIF4E. QLB1 revealed 7 alleles in a set of 100 winter barley lines and cultivars. Five alleles were found within 673 advanced breeding lines derived from applied Polish winter barley breeding programmes, which corresponds to a PIC value of 0.684. No recombinants between Rym4/5 and QLB1 were detected, suggesting that QLB1 can be used efficiently in marker-assisted selection of the Hv-eIF4E-mediated bymovirus resistance.
基因组序列AY661558代表了赋予大麦对大麦黄花叶病毒复合体（BaMMV/BaYMV）抗性的Rym4/Rym5基因座的BAC重叠群的一部分，为了开发用于实际大麦育种的SSR标记而对其进行了研究。在该序列中发现的57个SSR基序中，针对重复长度最长的5个SSR设计并测试了引物。多态性SSR标记QLB1在为构建原始BAC重叠群而开发的各自高分辨率作图群体中与rym4和rym5表型共分离。引物靶向位于翻译起始因子4E（Hv-eIF4E）下游756 bp和5173 bp的2个位点。在Morex BAC 519J14（Hv-eIF4E的种子BAC）和位于Hv-eIF4E近端的BAC 801A11上，通过实验证实了QLB1标记与Rym4/Rym5基因座的物理连锁。QLB1在一组100个冬大麦品系和品种中显示出7个等位基因。在来自波兰应用冬大麦育种计划的673个高级育种系中发现了5个等位基因，其多态信息含量（PIC）值为0.684。未检测到Rym4/5与QLB1之间的重组体，这表明QLB1可有效地用于由Hv-eIF4E介导的大麦黄花叶病毒抗性的标记辅助选择。
