Fiust Anna, Rapacz Marcin, Wójcik-Jagła Magdalena, Tyrka Mirosław
Department of Plant Physiology, University of Agriculture in Kraków, ul. Podłużna 3, 30-239, Kraków, Poland.
J Appl Genet. 2015 Aug;56(3):299-309. doi: 10.1007/s13353-015-0273-x. Epub 2015 Feb 26.
The tolerance of spring barley (Hordeum vulgare L.) cultivars to spring drought is an important agronomic trait affecting crop yield and quality in Poland. Therefore, breeders require new molecular markers to select plants with lower spring drought susceptibility. With the advent of genomic selection technology, simple molecular tools may still be applicable to screen material for markers of the most important traits and in-depth genome scanning. In previous studies, diversity arrays technology (DArT)-based genetic maps were constructed for F2 populations of Polish fodder and malt barley elite breeding lines, and 15 and 18 quantitative trait loci (QTLs) related to spring drought tolerance were identified, respectively. In this paper, we show the results of a conversion of 30 DArT markers corresponding to 11 QTLs into simple sequence repeat (SSR) and sequence tagged site (STS) markers. Twenty-two polymorphic markers were obtained, including 13 DArT-based SSRs. Additionally, 31 SSR markers, located in close proximity to the DArT markers, were selected from the GrainGenes database and tested. Further analyses of 24 advanced breeding lines with different drought tolerances confirmed that five out of the 30 converted markers, as well as three out of the 31 additional SSR markers, were effective in marker-assisted selection for drought tolerance. The possible function of clones related to these markers in drought tolerance is discussed.
春大麦（Hordeum vulgare L.）品种对春季干旱的耐受性是影响波兰作物产量和品质的重要农艺性状。因此，育种者需要新的分子标记来筛选对春季干旱敏感性较低的植株。随着基因组选择技术的出现，简单的分子工具可能仍适用于筛选最重要性状的标记以及进行深入的基因组扫描。在先前的研究中，为波兰饲料大麦和麦芽大麦优良育种系的F2群体构建了基于多样性阵列技术（DArT）的遗传图谱，分别鉴定出15个和18个与春季干旱耐受性相关的数量性状位点（QTL）。在本文中，我们展示了将对应于11个QTL的30个DArT标记转化为简单序列重复（SSR）和序列标签位点（STS）标记的结果。获得了22个多态性标记，包括13个基于DArT的SSR标记。此外，从GrainGenes数据库中选择并测试了31个与DArT标记紧密相邻的SSR标记。对24个具有不同耐旱性的高级育种系的进一步分析证实，30个转化标记中的5个以及31个额外SSR标记中的3个在耐旱性的标记辅助选择中是有效的。还讨论了与这些标记相关的克隆在耐旱性中的可能功能。
