Lindsey Wesley C, Woodruff Evelyn S, Weed Diane, Ward David C, Jenison Robert D
Inverness Medical Innovations, Inc., Louisville, CO 80027, USA.
Diagn Microbiol Infect Dis. 2008 Jul;61(3):273-9. doi: 10.1016/j.diagmicrobio.2008.03.002. Epub 2008 Apr 28.
We describe here a rapid assay for the detection of the tuf gene for the identification of Staphylococcus genus, the femB gene for the identification of Staphylococcus aureus species, and the mecA gene for the identification of methicillin resistance directly from BACTEC blood culture bottles showing Gram-positive cocci in clusters. The test, configured on a thin-film biosensor platform, allows for detection of genomic DNA from blood culture samples without the need for nucleic acid amplification. In an initial study to validate the technology, 107 consecutive positive blood cultures were tested on the thin-film biosensor, and the assay exhibited 100% concordance in comparison with standard microbiological methods for identifying methicillin-susceptible and methicillin-resistant S. aureus and for identifying methicillin-susceptible and methicillin-resistant coagulase-negative Staphylococcus. Results were obtained within 90 min directly from signal positive bottles with no instrumentation required.
我们在此描述一种快速检测方法，可直接从显示革兰氏阳性球菌呈簇状的BACTEC血培养瓶中检测用于鉴定葡萄球菌属的tuf基因、用于鉴定金黄色葡萄球菌种的femB基因以及用于鉴定耐甲氧西林的mecA基因。该检测方法配置在薄膜生物传感器平台上，无需核酸扩增即可检测血培养样本中的基因组DNA。在一项验证该技术的初步研究中，对107份连续的阳性血培养样本在薄膜生物传感器上进行了检测，与标准微生物学方法相比，该检测方法在鉴定甲氧西林敏感和耐甲氧西林的金黄色葡萄球菌以及鉴定甲氧西林敏感和耐甲氧西林的凝固酶阴性葡萄球菌方面表现出100%的一致性。直接从信号阳性的培养瓶中在90分钟内获得结果，无需任何仪器设备。
