p55识别组蛋白H4的结构基础。
Structural basis of histone H4 recognition by p55.
作者信息
Song Ji-Joon, Garlick Joseph D, Kingston Robert E
机构信息
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
出版信息
Genes Dev. 2008 May 15;22(10):1313-8. doi: 10.1101/gad.1653308. Epub 2008 Apr 28.
Abstract
p55 is a common component of many chromatin-modifying complexes and has been shown to bind to histones. Here, we present a crystal structure of Drosophila p55 bound to a histone H4 peptide. p55, a predicted WD40 repeat protein, recognizes the first helix of histone H4 via a binding pocket located on the side of a beta-propeller structure. The pocket cannot accommodate the histone fold of H4, which must be altered to allow p55 binding. Reconstitution experiments show that the binding pocket is important to the function of p55-containing complexes. These data demonstrate that WD40 repeat proteins use various surfaces to direct the modification of histones.
摘要
p55是许多染色质修饰复合物的常见组分，并且已被证明能与组蛋白结合。在此，我们展示了与组蛋白H4肽结合的果蝇p55的晶体结构。p55是一种预测的WD40重复蛋白，它通过位于β-螺旋桨结构一侧的结合口袋识别组蛋白H4的第一个螺旋。该口袋无法容纳H4的组蛋白折叠结构，H4的组蛋白折叠结构必须改变才能允许p55结合。重组实验表明，该结合口袋对含p55的复合物的功能很重要。这些数据证明，WD40重复蛋白利用各种表面来指导组蛋白的修饰。
相似文献
1
Structural basis of histone H4 recognition by p55.p55识别组蛋白H4的结构基础。
Genes Dev. 2008 May 15;22(10):1313-8. doi: 10.1101/gad.1653308. Epub 2008 Apr 28.
2
Chromatin-modifying complex component Nurf55/p55 associates with histones H3 and H4 and polycomb repressive complex 2 subunit Su(z)12 through partially overlapping binding sites.染色质修饰复合物成分 Nurf55/p55 通过部分重叠的结合位点与组蛋白 H3 和 H4 以及多梳抑制复合物 2 亚基 Su(z)12 结合。
J Biol Chem. 2011 Jul 1;286(26):23388-96. doi: 10.1074/jbc.M110.207407. Epub 2011 May 5.
3
The biological function of the WD40 repeat-containing protein p55/Caf1 in Drosophila.WD40 重复蛋白 p55/Caf1 在果蝇中的生物学功能。
Dev Dyn. 2012 Mar;241(3):455-64. doi: 10.1002/dvdy.23730. Epub 2012 Jan 12.
4
Diverse functions of WD40 repeat proteins in histone recognition.WD40重复蛋白在组蛋白识别中的多种功能。
Genes Dev. 2008 May 15;22(10):1265-8. doi: 10.1101/gad.1676208.
5
Structural basis for the recognition of histone H4 by the histone-chaperone RbAp46.组蛋白伴侣RbAp46识别组蛋白H4的结构基础。
Structure. 2008 Jul;16(7):1077-85. doi: 10.1016/j.str.2008.05.006. Epub 2008 Jun 19.
6
Structural insight into the interaction between the p55 PDZ domain and glycophorin C.对p55 PDZ结构域与血型糖蛋白C相互作用的结构洞察。
Biochem Biophys Res Commun. 2007 Aug 10;359(4):972-8. doi: 10.1016/j.bbrc.2007.05.215. Epub 2007 Jun 11.
7
Methods for analysis of nucleosome sliding by Drosophila NURF.果蝇NURF分析核小体滑动的方法。
Methods Enzymol. 2004;377:353-63. doi: 10.1016/S0076-6879(03)77022-0.
8
Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail.与赖氨酸9-甲基化组蛋白H3尾巴结合的HP1染色质结构域的结构。
Science. 2002 Mar 15;295(5562):2080-3. doi: 10.1126/science.1069473. Epub 2002 Feb 21.
9
p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes.p55是RbAp46/RbAp48在果蝇中的同源物，是抑制dE2F2/RBF调控基因所必需的。
Mol Cell Biol. 2004 Oct;24(20):9124-36. doi: 10.1128/MCB.24.20.9124-9136.2004.
10
Drosophila NURF-55, a WD repeat protein involved in histone metabolism.果蝇NURF-55，一种参与组蛋白代谢的WD重复蛋白。
Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):132-7. doi: 10.1073/pnas.95.1.132.
引用本文的文献
1
Deciphering the Molecular Mechanisms of BPTF Interactions with Nucleosomes via Molecular Simulations.通过分子模拟解析BPTF与核小体相互作用的分子机制
bioRxiv. 2025 Jun 14:2025.02.25.640153. doi: 10.1101/2025.02.25.640153.
2
Deciphering the molecular mechanisms of BPTF interactions with nucleosomes via molecular simulations.通过分子模拟解析BPTF与核小体相互作用的分子机制。
Biophys J. 2025 Jul 3. doi: 10.1016/j.bpj.2025.06.042.
3
Genome-wide identification of CaWD40 proteins reveals the involvement of a novel complex (CaAN1-CaDYT1-CaWD40-91) in anthocyanin biosynthesis and genic male sterility in Capsicum annuum.全基因组鉴定 CaWD40 蛋白揭示了一个新的复合物（CaAN1-CaDYT1-CaWD40-91）在辣椒花色苷生物合成和基因雄性不育中的作用。
BMC Genomics. 2024 Sep 11;25(1):851. doi: 10.1186/s12864-024-10681-9.
4
Genome-wide identification and analysis of WD40 proteins reveal that NtTTG1 enhances drought tolerance in tobacco (Nicotiana tabacum).全基因组鉴定和分析 WD40 蛋白表明，NtTTG1 增强了烟草（Nicotiana tabacum）的耐旱性。
BMC Genomics. 2024 Feb 2;25(1):133. doi: 10.1186/s12864-024-10022-w.
5
Histone Readers and Their Roles in Cancer.组蛋白读取器及其在癌症中的作用。
Cancer Treat Res. 2023;190:245-272. doi: 10.1007/978-3-031-45654-1_8.
6
Structural basis of nucleosome deacetylation and DNA linker tightening by Rpd3S histone deacetylase complex.Rpd3S 组蛋白去乙酰化酶复合物介导核小体去乙酰化和 DNA 连接子紧缩的结构基础。
Cell Res. 2023 Oct;33(10):790-801. doi: 10.1038/s41422-023-00869-1. Epub 2023 Sep 4.
7
The Role of Chromatin Assembly Factors in Induced Mutagenesis at Low Levels of DNA Damage.染色质组装因子在低水平 DNA 损伤诱导突变中的作用。
Genes (Basel). 2023 Jun 10;14(6):1242. doi: 10.3390/genes14061242.
8
Modulation of EZH2 Activity Induces an Antitumoral Effect and Cell Redifferentiation in Anaplastic Thyroid Cancer.EZH2 活性的调节可诱导间变性甲状腺癌的抗肿瘤作用和细胞再分化。
Int J Mol Sci. 2023 Apr 26;24(9):7872. doi: 10.3390/ijms24097872.
9
Caf1 regulates the histone methyltransferase activity of Ash1 by sensing unmodified histone H3.Caf1 通过感应未修饰的组蛋白 H3 来调节 Ash1 的组蛋白甲基转移酶活性。
Epigenetics Chromatin. 2023 Apr 29;16(1):15. doi: 10.1186/s13072-023-00487-6.
10
Genome-wide identification of WD40 transcription factors and their regulation of the MYB-bHLH-WD40 (MBW) complex related to anthocyanin synthesis in Qingke (Hordeum vulgare L. var. nudum Hook. f.).全基因组鉴定 WD40 转录因子及其对与青稞（裸大麦，Hordeum vulgare L. var. nudum Hook. f.）花色苷合成相关的 MYB-bHLH-WD40（MBW）复合物的调控。
BMC Genomics. 2023 Apr 4;24(1):166. doi: 10.1186/s12864-023-09240-5.
本文引用的文献
1
Chromatin challenges during DNA replication and repair.DNA复制和修复过程中的染色质挑战。
Cell. 2007 Feb 23;128(4):721-33. doi: 10.1016/j.cell.2007.01.030.
2
The role of chromatin during transcription.染色质在转录过程中的作用。
Cell. 2007 Feb 23;128(4):707-19. doi: 10.1016/j.cell.2007.01.015.
3
Structural basis for molecular recognition and presentation of histone H3 by WDR5.WDR5对组蛋白H3进行分子识别和呈递的结构基础。
EMBO J. 2006 Sep 20;25(18):4245-52. doi: 10.1038/sj.emboj.7601316. Epub 2006 Aug 31.
4
Molecular recognition of histone H3 by the WD40 protein WDR5.WD40蛋白WDR5对组蛋白H3的分子识别
Nat Struct Mol Biol. 2006 Aug;13(8):698-703. doi: 10.1038/nsmb1116. Epub 2006 Jul 9.
5
Histone H3 recognition and presentation by the WDR5 module of the MLL1 complex.MLL1复合物的WDR5模块对组蛋白H3的识别与呈递。
Nat Struct Mol Biol. 2006 Aug;13(8):704-12. doi: 10.1038/nsmb1119. Epub 2006 Jul 9.
6
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling.NURF的一个PHD指结构域将组蛋白H3赖氨酸4三甲基化与染色质重塑联系起来。
Nature. 2006 Jul 6;442(7098):86-90. doi: 10.1038/nature04815. Epub 2006 May 21.
7
Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5.WD-40蛋白WDR5对甲基化组蛋白H3赖氨酸4特异性识别的结构基础。
Mol Cell. 2006 Apr 7;22(1):137-44. doi: 10.1016/j.molcel.2006.03.018.
8
Regulation of flowering time by Arabidopsis MSI1.拟南芥MSI1对开花时间的调控
Development. 2006 May;133(9):1693-702. doi: 10.1242/dev.02340. Epub 2006 Mar 22.
9
WDR5 associates with histone H3 methylated at K4 and is essential for H3 K4 methylation and vertebrate development.WD重复结构域5（WDR5）与在赖氨酸4（K4）处发生甲基化的组蛋白H3相关联，并且对于H3 K4甲基化和脊椎动物发育至关重要。
Cell. 2005 Jun 17;121(6):859-72. doi: 10.1016/j.cell.2005.03.036.
10
Loss of function of MULTICOPY SUPPRESSOR OF IRA 1 produces nonviable parthenogenetic embryos in Arabidopsis.IRA1多拷贝抑制子功能丧失会导致拟南芥中孤雌生殖胚胎无法存活。
Curr Biol. 2005 Apr 26;15(8):750-4. doi: 10.1016/j.cub.2005.02.066.
Zotero 插件