Guller Seth, Ma Yula Y, Fu Han-Hsuan, Krikun Graciela, Abrahams Vikki M, Mor Gil
Department of Obstetrics/Gynecology, Yale University School of Medicine, 333 Cedar Street, 339 FMB, P.O. Box 208063, New Haven, CT 06520-8063, USA.
Ann N Y Acad Sci. 2008 Apr;1127:129-33. doi: 10.1196/annals.1434.015.
Preeclampsia is associated with an increased release of factors from the placental syncytium into maternal blood, including the antiangiogenic factors soluble fms-like tyrosine kinase-1 and soluble endoglin, the antifibrinolytic factor plasminogen activator inhibitor-1, prostanoids, lipoperoxides, cytokines, and microparticles. These factors are suggested to promote maternal endothelium dysfunction and are associated with placental damage in pregnancies also complicated with intrauterine growth restriction (IUGR). In this report, we briefly describe the interaction of syncytial factors with hypoxia, reactive oxygen species, and apoptosis in the pathophysiology of preeclampsia and IUGR. Given the critical role of the syncytium in these complications of pregnancy, we also present a novel methodology in which laser capture microdissection followed by Western blotting is used to assess levels of syncytial Fas ligand, a key protein in the apoptotic cascade.
子痫前期与胎盘合体滋养层释放到母体血液中的多种因子增加有关，这些因子包括抗血管生成因子可溶性fms样酪氨酸激酶-1和可溶性内皮糖蛋白、抗纤溶因子纤溶酶原激活物抑制剂-1、前列腺素、脂过氧化物、细胞因子和微粒。这些因子被认为会促进母体血管内皮功能障碍，并且与同时合并胎儿生长受限（IUGR）的妊娠中的胎盘损伤有关。在本报告中，我们简要描述了合体滋养层因子在子痫前期和胎儿生长受限病理生理学中与缺氧、活性氧和细胞凋亡的相互作用。鉴于合体滋养层在这些妊娠并发症中的关键作用，我们还介绍了一种新方法，即采用激光捕获显微切割技术，随后进行蛋白质印迹法，以评估凋亡级联反应中的关键蛋白——合体滋养层Fas配体的水平。
