分析型表面增强拉曼光谱和多光谱成像的细胞内应用。

Intracellular applications of analytical SERS spectroscopy and multispectral imaging.

作者信息

Chourpa Igor, Lei Franck H, Dubois Pierre, Manfait Michel, Sockalingum Ganesh D

机构信息

Université François-Rabelais, Faculté de Pharmacie, Focalisation magnétique d'agents anticancéreux, 31 avenue Monge, F-37200 Tours, France.

出版信息

Chem Soc Rev. 2008 May;37(5):993-1000. doi: 10.1039/b714732p. Epub 2008 Mar 26.

DOI:10.1039/b714732p

PMID:18443684

Abstract

The aim of this tutorial review is to give an overview of the state of the art of intracellular applications of analytical SERS spectroscopy. We pay particular attention to nanoparticle-based SERS spectroscopy since this currently dominates the published literature on non-disturbing analysis of live cells. We describe recent advances in this domain due to the development of multispectral imaging and to the combined use of SERRS (surface-enhanced resonance Raman scattering) and fluorescence spectroscopy. Finally, a perspective view is given on the tip-based approaches like tip-enhanced Raman spectroscopy (TERS) which allow micrometric and nanometric resolution.

摘要

本教程综述的目的是概述分析型表面增强拉曼光谱（SERS）在细胞内应用的最新技术水平。我们特别关注基于纳米颗粒的SERS光谱，因为目前它在关于活细胞非侵入性分析的已发表文献中占主导地位。我们描述了由于多光谱成像的发展以及表面增强共振拉曼散射（SERRS）与荧光光谱的联合使用而在该领域取得的最新进展。最后，对基于探针的方法，如允许微米和纳米级分辨率的针尖增强拉曼光谱（TERS），给出了一个前瞻性的观点。