Schlücker Sebastian
Department of Physics, University of Osnabrück, D-49069 Osnabrück, Germany.
Chemphyschem. 2009 Jul 13;10(9-10):1344-54. doi: 10.1002/cphc.200900119.
Surface-enhanced Raman scattering (SERS) microscopy is a novel method of vibrational microspectroscopic imaging for the selective detection of biomolecules in targeted research. This technique combines the advantages of biofunctionalized metal nanoparticles and Raman microspectroscopy for visualizing and quantifying the distribution of target molecules such as proteins in cells and tissues. Advantages of SERS over existing labeling approaches include the tremendous multiplexing capacity, quantification using the characteristic SERS signatures and high photostability. This review summarizes current designs of nanoparticle-based SERS probes and highlights first biomedical applications of SERS microscopy for protein localization ex and in vivo.
表面增强拉曼散射(SERS)显微镜是一种用于靶向研究中生物分子选择性检测的新型振动显微光谱成像方法。该技术结合了生物功能化金属纳米颗粒和拉曼显微光谱的优势,用于可视化和定量细胞及组织中蛋白质等靶分子的分布。与现有标记方法相比,SERS的优势包括巨大的多重检测能力、利用特征SERS信号进行定量以及高光稳定性。本文综述了基于纳米颗粒的SERS探针的当前设计,并重点介绍了SERS显微镜在体内外蛋白质定位方面的首次生物医学应用。