Varshavsky A J, Bakayev V V, Chumackov P M, Georgiev G P
Nucleic Acids Res. 1976 Aug;3(8):2101-13. doi: 10.1093/nar/3.8.2101.
In contrast to conclusions of previous studies /I-3/ claiming the absence of histone HI from the SV40 and polyoma viral minichromosomes we have found that a preparation of purified SV40 minichromosomes does contain histone HI. The content of HI in relation to other four histones in the SV40 minichromosomes is close to that in the cellular chromatin. Histone HI in the isolated SV40 minichromosomes is bound apparently to internucleosomal DNA stretches as was shown already for HI in the cellular chromatin /4/. In addition it was found that more than 90% of the purified SV40 minichromosomes migrated as a single discrete deoxyribonucleoprotein band upon agarose gel electrophoresis.
与之前声称SV40和多瘤病毒微型染色体中不存在组蛋白H1的研究/I-3/的结论相反，我们发现纯化的SV40微型染色体制剂确实含有组蛋白H1。SV40微型染色体中H1与其他四种组蛋白的含量与细胞染色质中的含量相近。分离出的SV40微型染色体中的组蛋白H1显然与核小体间的DNA片段结合，这在细胞染色质中的H1中已得到证实/4/。此外，还发现超过90%的纯化SV40微型染色体在琼脂糖凝胶电泳中作为单一离散的脱氧核糖核蛋白带迁移。
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