Bakaev V V, Nedospasov S A, Georgiev G P
Mol Biol (Mosk). 1981 Jul-Aug;15(4):939-49.
Extraction of the purified nuclei of SV40 infected cells reveals a heterogeneous set of viral DNA-protein complexes. Earlier, the authors have shown the possibility of nuclear particles extraction being indistinguishable from mature SV40 virions. In the present work, structural intermediates of virus maturation from free minichromosomes through replicative complexes to immature virion particles have been analyzed. The fractionation of viral complexes by non-denaturing agarose gel electrophoresis has been employed. The protein composition of the complexes as determined by two-dimensional gel electrophoresis indicates that five histone fractions including H1 are present during minichromosome maturation to the chromosome of the mature virion.
对感染SV40的细胞进行纯化细胞核提取，结果显示出一组异质性的病毒DNA - 蛋白质复合物。此前，作者已经表明，提取的核颗粒可能与成熟的SV40病毒粒子无法区分。在本研究中，分析了病毒从游离的微型染色体通过复制复合物到未成熟病毒粒子的成熟结构中间体。采用非变性琼脂糖凝胶电泳对病毒复合物进行分级分离。通过二维凝胶电泳确定的复合物蛋白质组成表明，在微型染色体成熟为成熟病毒粒子染色体的过程中，存在包括H1在内的五个组蛋白组分。
