Mahata Julie, Argos Maria, Verret Wendy, Kibriya Muhammad G, Santella Regina M, Ahsan Habibul
Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY, USA.
Nutr Cancer. 2008;60(1):55-60. doi: 10.1080/01635580701761282.
An estimated 35 million people in Bangladesh have been chronically exposed to arsenic in drinking water and are at risk of an array of adverse health conditions. The mechanisms of arsenic toxicity have not been well established; however, oxidative stress has been one commonly proposed pathway. In this study, we evaluated the effect of antioxidant supplementation on plasma protein oxidation among patients with arsenical skin lesions participating in a randomized double-blinded placebo-controlled trial of vitamin E and selenium. Subjects were randomized to 1 of 4 treatments arms (vitamin E, selenium, combination, or placebo) and were treated for a 6-mo period. We observed a dose-dependent increase in adjusted protein carbonyl levels by arsenic exposure status in the pretreatment samples, although trends were not statistically significant. Following the 6-mo intervention, there was a decrease in protein carbonyl levels in each treatment group, although no resultant decrease was significantly different from that seen in the placebo group. Although we did not see a notable effect of selenium or vitamin E supplementation on changes in protein carbonyl levels, these preliminary data demonstrate a feasible methodological approach for the assessment of plasma protein carbonyls in relation to environmental toxicants in a human population and their potential use as endpoints in intervention trials.
据估计，孟加拉国有3500万人长期饮用含砷的水，面临一系列健康问题的风险。砷中毒的机制尚未完全明确；然而，氧化应激是一个普遍提出的途径。在这项研究中，我们评估了抗氧化剂补充对参与维生素E和硒随机双盲安慰剂对照试验的砷性皮肤病变患者血浆蛋白氧化的影响。受试者被随机分为4个治疗组之一（维生素E、硒、联合用药或安慰剂），并接受为期6个月的治疗。我们观察到，在预处理样本中，根据砷暴露状况，调整后的蛋白羰基水平呈剂量依赖性增加，尽管趋势无统计学意义。经过6个月的干预，每个治疗组的蛋白羰基水平均有所下降，尽管没有一个治疗组的下降幅度与安慰剂组有显著差异。虽然我们没有看到补充硒或维生素E对蛋白羰基水平变化有显著影响，但这些初步数据证明了一种可行的方法，可用于评估人群中与环境毒物相关的血浆蛋白羰基，以及它们作为干预试验终点的潜在用途。
