对合成细胞外基质注射至声带固有层的炎性细胞因子反应。
Inflammatory cytokine responses to synthetic extracellular matrix injection to the vocal fold lamina propria.
作者信息
Thibeault Susan L, Duflo Suzy
机构信息
Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison 53792-7375, USA.
出版信息
Ann Otol Rhinol Laryngol. 2008 Mar;117(3):221-6. doi: 10.1177/000348940811700310.
OBJECTIVES
We determined cytokine profiles for tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, IL-1alpha, and IL-1beta after injection of a synthetic extracellular matrix into the vocal fold lamina propria.
METHODS
Transcript expression of inflammatory cytokines was measured with real-time polymerase chain reaction on postoperative days 1, 3, 5, 10, and 21 in 25 rabbits that underwent bilateral vocal fold biopsy with Extracel-LG (Carbylan-GSX 5%) immediately injected into the wound in the left vocal fold and saline solution injected into the wound in the right vocal fold. Two unwounded normal rabbit larynges were also harvested for controls.
RESULTS
Significantly elevated levels of TNF-alpha, IL-1beta, and IL-6 were measured on day 3 in a comparison between Extracel-LG-injected vocal folds and saline solution-injected control vocal folds and between Extracel-LG-injected vocal folds and normal vocal folds. All cytokine levels returned to baseline by day 21.
CONCLUSIONS
A mild short-term inflammatory response was measured early after injection of a synthetic extracellular matrix into the vocal fold lamina propria.
目的
我们测定了在向声带固有层注射合成细胞外基质后，肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6、IL-1α和IL-1β的细胞因子谱。
方法
对25只接受双侧声带活检的兔子，在术后第1、3、5、10和21天，用实时聚合酶链反应测量炎性细胞因子的转录表达。这些兔子中，左侧声带伤口立即注射Extracel-LG（Carbylan-GSX 5%），右侧声带伤口注射盐溶液。还获取了两个未受伤的正常兔喉作为对照。
结果
在注射Extracel-LG的声带与注射盐溶液的对照声带之间，以及注射Extracel-LG的声带与正常声带之间的比较中，第3天测量到TNF-α、IL-1β和IL-6水平显著升高。所有细胞因子水平在第21天恢复到基线。
结论
在向声带固有层注射合成细胞外基质后早期，检测到轻度短期炎症反应。
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