一种用于博来霉素的新型DNA发夹底物。
A novel DNA hairpin substrate for bleomycin.
作者信息
Akiyama Yoshitsugu, Ma Qian, Edgar Erin, Laikhter Andrei, Hecht Sidney M
机构信息
Department of Chemistry and Biology, University of Virginia, Charlottesville, Virginia 22904, USA.
出版信息
Org Lett. 2008 Jun 5;10(11):2127-30. doi: 10.1021/ol800445x. Epub 2008 Apr 30.
A 16-nucleotide DNA hairpin containing 4-aminobenzo[g]quinazoline-2-one 2'-deoxyribose at position 15 has been prepared and found to lack significant fluorescence. When treated with Fe(II).BLM, the hairpin was found to undergo oxidative transformation selectively at position 15. The predominant fluorescent product was characterized and quantified. The pro-fluorescent DNA hairpin was used as a substrate for 15 bleomycin congeners, and the results were compared with those obtained following cleavage of a radiolabeled DNA duplex and PAGE analysis.
已制备出一种在第15位含有4-氨基苯并[g]喹唑啉-2-酮2'-脱氧核糖的16核苷酸DNA发夹结构，发现其缺乏显著荧光。用Fe(II).博来霉素处理后，发现该发夹结构在第15位选择性地发生氧化转化。对主要的荧光产物进行了表征和定量。将这种前荧光DNA发夹用作15种博来霉素类似物的底物，并将结果与放射性标记的DNA双链体切割及聚丙烯酰胺凝胶电泳分析后得到的结果进行比较。
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