Liu Yichun, Zhong Mingya, Shan Guiye, Li Yajun, Huang Baiqu, Yang Guoliang
Centre for Advanced Optoelectronic Functional Materials Research, Institute of Genetics and Cytology, Northeast Normal University, Changchun, People's Republic of China.
J Phys Chem B. 2008 May 22;112(20):6484-9. doi: 10.1021/jp710399d. Epub 2008 Apr 30.
A novel method for identifying DNA microarrays based on ZnO/Au nanocomposites functionalized with thiol-oligonucleotide as probes is descried here. DNA labeled with ZnO/Au nanocomposites has a strong Raman signal even without silver acting as a surface-enhanced Raman scattering promoter. X-ray photoelectron spectra confirmed the formation of a three-component sandwich assay, i.e., constituted DNA and ZnO/Au nanocomposites. The resonance multiple-phonon Raman signal of the ZnO/Au nanocomposites as a spectroscopic fingerprint is used to detect a target sequence of oligonucleotide. This method exhibits extraordinary sensitivity and the detection limit is at least 1 fM.
本文描述了一种基于用硫醇-寡核苷酸功能化的ZnO/Au纳米复合材料作为探针来识别DNA微阵列的新方法。即使没有银作为表面增强拉曼散射促进剂，用ZnO/Au纳米复合材料标记的DNA也具有很强的拉曼信号。X射线光电子能谱证实了三组分夹心分析的形成，即由DNA和ZnO/Au纳米复合材料构成。ZnO/Au纳米复合材料的共振多声子拉曼信号作为光谱指纹用于检测寡核苷酸的靶序列。该方法具有极高的灵敏度，检测限至少为1 fM。
