Vorbeck M, Oswald T, Martin A
Prep Biochem. 1976;6(5):387-402. doi: 10.1080/00327487608061626.
The PolytronR and Dounce homogenizers have been evaluated for preparation of homogenates of rat liver prior to isolation of subcellular fractions by differential centrifugation. Marker enzymes used to evaluate the subcellular fractions included cytochrome oxidase, monoamine oxidase, D-amino acid oxidase, acid phosphatase, glucose-6-phosphatase, ethyl morphine demethylase, and lactate dehydrogenase. No significant difference in the distribution of enzymes (percent recovery or specific activity) was observed between the two methods of homogenization. In addition, there were no significant differences in the ultrastructural appearances and respiratory control ratios of the mitochondrial fractions prepared by the two methods of homogenization.
在通过差速离心分离亚细胞组分之前，已对PolytronR匀浆器和Dounce匀浆器用于制备大鼠肝脏匀浆进行了评估。用于评估亚细胞组分的标记酶包括细胞色素氧化酶、单胺氧化酶、D-氨基酸氧化酶、酸性磷酸酶、葡萄糖-6-磷酸酶、乙基吗啡脱甲基酶和乳酸脱氢酶。两种匀浆方法之间在酶的分布（回收率百分比或比活性）上未观察到显著差异。此外，两种匀浆方法制备的线粒体组分在超微结构外观和呼吸控制率方面也没有显著差异。
