Baker Neil R
Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, United Kingdom.
Annu Rev Plant Biol. 2008;59:89-113. doi: 10.1146/annurev.arplant.59.032607.092759.
The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO(2) assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO(2) assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered.
叶绿素荧光用于监测藻类和植物光合性能的方法如今已广泛应用。本综述探讨了荧光参数如何用于评估体内光系统II（PSII）光化学、线性电子流和CO₂同化作用的变化，并概述了使用特定荧光参数的理论基础。尽管荧光参数易于测量，但在应用它们来预测光合性能变化时可能会出现许多潜在问题。特别要考虑的是与通过荧光测量的PSII运行效率的准确估计及其与线性电子流速率和CO₂同化作用速率的关系相关的问题。研究了光化学猝灭和非光化学猝灭在确定PSII运行效率变化中的作用。最后，考虑了荧光成像在光合异质性研究以及对大量植物进行光合作用和相关代谢扰动快速筛选方面的应用。
