• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

小鼠促性腺激素释放激素神经元中CRE重组酶表达的时空调控

Temporal and spatial regulation of CRE recombinase expression in gonadotrophin-releasing hormone neurones in the mouse.

作者信息

Wolfe A, Divall S, Singh S P, Nikrodhanond A A, Baria A T, Le W W, Hoffman G E, Radovick S

机构信息

Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.

出版信息

J Neuroendocrinol. 2008 Jul;20(7):909-16. doi: 10.1111/j.1365-2826.2008.01746.x. Epub 2008 Apr 28.

DOI:10.1111/j.1365-2826.2008.01746.x
PMID:18445125
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2658716/
Abstract

Gonadotrophin-releasing hormone (GnRH) neurones located within the brain are the final neuroendocrine output regulating the reproductive hormone axis. Their small number and scattered distribution in the hypothalamus make them particularly difficult to study in vivo. The Cre/loxP system is a valuable tool to delete genes in specific cells and tissues. We report the production of two mouse lines that express the CRE bacteriophage recombinase in a GnRH-specific manner. The first line, the GnRH-CRE mouse, contains a transgene in which CRE is under the control of the murine GnRH promoter and targets CRE expression specifically to GnRH neurones in the hypothalamus. The second line, the GnRH-CRETeR mouse, uses the same murine GnRH promoter to target CRE expression to GnRH neurones, but is modified to be constitutively repressed by a tetracycline repressor (TetR) expressed from a downstream tetracycline repressor gene engineered within the transgene. GnRH neurone-specific CRE expression can therefore be induced by treatment with doxycycline which relieves repression by TetR. These GnRH-CRE and GnRH-CRETeR mice can be used to study the function of genes expressed specifically in GnRH neurones. The GnRH-CRETeR mouse can be used to study genes that may have distinct roles in reproductive physiology during the various developmental stages.

摘要

位于大脑内的促性腺激素释放激素（GnRH）神经元是调节生殖激素轴的最终神经内分泌输出。它们数量少且分散分布于下丘脑，这使得在体内研究它们特别困难。Cre/loxP系统是在特定细胞和组织中删除基因的宝贵工具。我们报告了两种以GnRH特异性方式表达CRE噬菌体重组酶的小鼠品系的产生。第一个品系，即GnRH-CRE小鼠，含有一个转基因，其中CRE受小鼠GnRH启动子的控制，并将CRE表达特异性靶向到下丘脑的GnRH神经元。第二个品系，即GnRH-CRETeR小鼠，使用相同的小鼠GnRH启动子将CRE表达靶向到GnRH神经元，但经过修饰，可被转基因内工程化的下游四环素阻遏物（TetR）基因表达的四环素阻遏物组成性抑制。因此，GnRH神经元特异性CRE表达可以通过强力霉素处理来诱导，强力霉素可解除TetR的抑制作用。这些GnRH-CRE和GnRH-CRETeR小鼠可用于研究在GnRH神经元中特异性表达基因的功能。GnRH-CRETeR小鼠可用于研究在不同发育阶段可能在生殖生理学中具有不同作用的基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/347742461216/jne0020-0909-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/299a18a88bae/jne0020-0909-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/5caff277fc37/jne0020-0909-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/38eb818916cb/jne0020-0909-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/128c025110b6/jne0020-0909-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/347742461216/jne0020-0909-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/299a18a88bae/jne0020-0909-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/5caff277fc37/jne0020-0909-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/38eb818916cb/jne0020-0909-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/128c025110b6/jne0020-0909-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/caeb/2658716/347742461216/jne0020-0909-f5.jpg

相似文献

1
Temporal and spatial regulation of CRE recombinase expression in gonadotrophin-releasing hormone neurones in the mouse.小鼠促性腺激素释放激素神经元中CRE重组酶表达的时空调控
J Neuroendocrinol. 2008 Jul;20(7):909-16. doi: 10.1111/j.1365-2826.2008.01746.x. Epub 2008 Apr 28.
2
A novel technique for temporally regulated cell type-specific Cre expression and recombination in the pituitary gonadotroph.一种用于垂体促性腺细胞中时间调控的细胞类型特异性Cre表达和重组的新技术。
J Mol Endocrinol. 2006 Aug;37(1):63-9. doi: 10.1677/jme.1.02053.
3
Roles for primary cilia in gonadotrophin-releasing hormone neurones in the mouse.小鼠促性腺激素释放激素神经元中初级纤毛的作用。
J Neuroendocrinol. 2014 Jan;26(1):18-25. doi: 10.1111/jne.12122.
4
A tetracycline-inducible and skeletal muscle-specific Cre recombinase transgenic mouse.一种四环素诱导型且骨骼肌特异性的Cre重组酶转基因小鼠。
Dev Neurobiol. 2009 May;69(6):401-6. doi: 10.1002/dneu.20714.
5
Physiology of the gonadotrophin-releasing hormone (GnRH) neurone: studies from embryonic GnRH neurones.促性腺激素释放激素（GnRH）神经元的生理学：来自胚胎 GnRH 神经元的研究。
J Neuroendocrinol. 2011 Jun;23(6):542-53. doi: 10.1111/j.1365-2826.2011.02130.x.
6
Sexually dimorphic distribution of Prokr2 neurons revealed by the Prokr2-Cre mouse model.Prokr2-Cre 小鼠模型揭示 Prokr2 神经元的性别二态性分布。
Brain Struct Funct. 2017 Dec;222(9):4111-4129. doi: 10.1007/s00429-017-1456-5. Epub 2017 Jun 14.
7
Three-dimensional representation of the neurotransmitter systems of the human hypothalamus: inputs of the gonadotrophin hormone-releasing hormone neuronal system.人类下丘脑神经递质系统的三维表示：促性腺激素释放激素神经元系统的输入
J Neuroendocrinol. 2006 Feb;18(2):79-95. doi: 10.1111/j.1365-2826.2005.01398.x.
8
Differential CRE Expression in Lhrh-cre and GnRH-cre Alleles and the Impact on Fertility in Otx2-Flox Mice.Lhrh-cre 和 GnRH-cre 等位基因中的差异 CRE 表达及其对 Otx2-Flox 小鼠生育力的影响。
Neuroendocrinology. 2019;108(4):328-342. doi: 10.1159/000497791. Epub 2019 Feb 10.
9
Neuron-specific expression of Cre recombinase during the late phase of brain development.大脑发育后期Cre重组酶的神经元特异性表达。
Neurosci Res. 2001 Jun;40(2):125-32. doi: 10.1016/s0168-0102(01)00216-4.
10
Early development of forebrain gonadotrophin-releasing hormone (GnRH) neurones and the role of GnRH as an autocrine migration factor.前脑促性腺激素释放激素（GnRH）神经元的早期发育以及GnRH作为自分泌迁移因子的作用。
J Neuroendocrinol. 2008 Mar;20(3):394-405. doi: 10.1111/j.1365-2826.2008.01654.x. Epub 2008 Jan 17.

引用本文的文献

1
Differentiation Capacity of Bone Marrow-Derived Rat Mesenchymal Stem Cells from DsRed and Cre Transgenic Cre/ Models.DsRed 和 Cre 转基因 Cre/模型大鼠骨髓间充质干细胞的分化能力。
Cells. 2022 Sep 5;11(17):2769. doi: 10.3390/cells11172769.
2
Lower FSH With Normal Fertility in Male Mice Lacking Gonadotroph Kisspeptin Receptor.缺乏促性腺激素释放激素神经元 kisspeptin 受体的雄性小鼠中 FSH 降低但生育力正常
Front Physiol. 2022 Apr 26;13:868593. doi: 10.3389/fphys.2022.868593. eCollection 2022.
3
Development of the gonadotropin-releasing hormone system.

本文引用的文献

1
Definition of estrogen receptor pathway critical for estrogen positive feedback to gonadotropin-releasing hormone neurons and fertility.雌激素受体通路的定义对雌激素对促性腺激素释放激素神经元的正反馈及生育能力至关重要。
Neuron. 2006 Oct 19;52(2):271-80. doi: 10.1016/j.neuron.2006.07.023.
2
A novel technique for temporally regulated cell type-specific Cre expression and recombination in the pituitary gonadotroph.一种用于垂体促性腺细胞中时间调控的细胞类型特异性Cre表达和重组的新技术。
J Mol Endocrinol. 2006 Aug;37(1):63-9. doi: 10.1677/jme.1.02053.
3
Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre/loxP mediated recombination in the nervous system, kidney, and somite-derived tissues.
促性腺激素释放激素系统的发育。
J Neuroendocrinol. 2022 May;34(5):e13087. doi: 10.1111/jne.13087. Epub 2022 Jan 23.
4
Anatomical Markers of Activity in Hypothalamic Neurons.下丘脑神经元活动的解剖学标记。
Compr Physiol. 2020 Mar 12;10(2):549-575. doi: 10.1002/cphy.c170021.
5
The Homeodomain Transcription Factors Vax1 and Six6 Are Required for SCN Development and Function.Homeodomain 转录因子 Vax1 和 Six6 对于 SCN 的发育和功能是必需的。
Mol Neurobiol. 2020 Feb;57(2):1217-1232. doi: 10.1007/s12035-019-01781-9. Epub 2019 Nov 9.
6
Deletion of the Homeodomain Protein Six6 From GnRH Neurons Decreases GnRH Gene Expression, Resulting in Infertility.同源盒蛋白 Six6 从 GnRH 神经元中的缺失会降低 GnRH 基因的表达，导致不孕。
Endocrinology. 2019 Sep 1;160(9):2151-2164. doi: 10.1210/en.2019-00113.
7
The Contribution of the Circadian Gene to Female Fertility and the Generation of the Preovulatory Luteinizing Hormone Surge.昼夜节律基因对女性生育能力及排卵前促黄体生成素高峰的产生的作用。
J Endocr Soc. 2019 Feb 13;3(4):716-733. doi: 10.1210/js.2018-00228. eCollection 2019 Apr 1.
8
Differential CRE Expression in Lhrh-cre and GnRH-cre Alleles and the Impact on Fertility in Otx2-Flox Mice.Lhrh-cre 和 GnRH-cre 等位基因中的差异 CRE 表达及其对 Otx2-Flox 小鼠生育力的影响。
Neuroendocrinology. 2019;108(4):328-342. doi: 10.1159/000497791. Epub 2019 Feb 10.
9
Impairments in the reproductive axis of female mice lacking estrogen receptor β in GnRH neurons.缺乏 GnRH 神经元中雌激素受体 β 的雌性小鼠生殖轴受损。
Am J Physiol Endocrinol Metab. 2018 Nov 1;315(5):E1019-E1033. doi: 10.1152/ajpendo.00173.2018. Epub 2018 Jul 24.
10
Haploinsufficiency of SIX3 Abolishes Male Reproductive Behavior Through Disrupted Olfactory Development, and Impairs Female Fertility Through Disrupted GnRH Neuron Migration.SIX3 单倍不足通过破坏嗅觉发育消除雄性生殖行为，并通过破坏 GnRH 神经元迁移损害雌性生育能力。
Mol Neurobiol. 2018 Nov;55(11):8709-8727. doi: 10.1007/s12035-018-1013-0. Epub 2018 Mar 27.
巢蛋白-Cre转基因小鼠品系Nes-Cre1在神经系统、肾脏和体节衍生组织中介导高效的Cre/loxP介导的重组。
Genesis. 2006 Aug;44(8):355-60. doi: 10.1002/dvg.20226.
4
Olfactory inputs to hypothalamic neurons controlling reproduction and fertility.嗅觉对控制生殖和生育能力的下丘脑神经元的输入。
Cell. 2005 Nov 18;123(4):669-82. doi: 10.1016/j.cell.2005.08.039.
5
Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons.下丘脑和隔区神经元中缺乏含 GluR-B 的 AMPA 受体而非 NMDA 受体导致生殖行为受损。
Mol Endocrinol. 2006 Jan;20(1):219-31. doi: 10.1210/me.2005-0262. Epub 2005 Aug 11.
6
Strategies of conditional gene expression in myocardium: an overview.心肌中条件性基因表达的策略：综述
Methods Mol Med. 2005;112:109-54. doi: 10.1007/978-1-59259-879-3_8.
7
Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract.发育中的肾脏和泌尿生殖道中的上皮特异性Cre/lox重组。
J Am Soc Nephrol. 2002 Jul;13(7):1837-46. doi: 10.1097/01.asn.0000016444.90348.50.
8
Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse.通过他莫昔芬诱导型Cre在多种组织中实现高效重组：一种用于小鼠中时间调控基因激活/失活的工具。
Dev Biol. 2002 Apr 15;244(2):305-18. doi: 10.1006/dbio.2002.0597.
9
beta-cell-specific deletion of the Igf1 receptor leads to hyperinsulinemia and glucose intolerance but does not alter beta-cell mass.胰岛β细胞特异性缺失胰岛素样生长因子1受体导致高胰岛素血症和葡萄糖不耐受，但不改变β细胞质量。
Nat Genet. 2002 May;31(1):111-5. doi: 10.1038/ng872. Epub 2002 Apr 1.
10
Codon-improved Cre recombinase (iCre) expression in the mouse.密码子优化的Cre重组酶（iCre）在小鼠中的表达。
Genesis. 2002 Jan;32(1):19-26. doi: 10.1002/gene.10023.