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Lowry蛋白质测定法的显色时间。

Color development time of the Lowry protein assay.

作者信息

Pomory Christopher M

机构信息

Department of Biology, University of West Florida, Pensacola, FL 32514, USA.

出版信息

Anal Biochem. 2008 Jul 15;378(2):216-7. doi: 10.1016/j.ab.2008.04.015. Epub 2008 Apr 10.

DOI:10.1016/j.ab.2008.04.015
PMID:18448065
Abstract

Color development of the Lowry protein assay was tracked over time for bovine serum albumin (BSA) concentrations ranging from 40 to 600 microg/ml. The time interval between 2 and 4h produced the most stable readings. This time frame also improved linearity of the standard curve.

摘要

在40至600微克/毫升的牛血清白蛋白(BSA)浓度范围内,随时间跟踪了Lowry蛋白质测定法的显色情况。2至4小时的时间间隔产生了最稳定的读数。这个时间段也改善了标准曲线的线性。

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