Zhang Guo Qing, Guan Ya Yi, Sheng Hai Hui, Zheng Bin, Wu Song, Xiao Hua Sheng, Tang Lin Hua
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis, and Filariasis, 207 Rui Jin Er Road, Shanghai 200025, China.
J Clin Microbiol. 2008 Jul;46(7):2167-74. doi: 10.1128/JCM.00081-08. Epub 2008 Apr 30.
Drug resistance in Plasmodium falciparum is a serious public health threat in the countries where this organism is endemic. Since resistance has been associated with specific single-nucleotide polymorphisms (SNPs) in parasite genes, molecular markers are becoming useful surrogates for monitoring the emergence and dispersion of drug resistance. In this study, a multiplex PCR (mPCR) and oligonucleotide microarray method was developed for the detection of these SNPs in genes encoding chloroquine resistance transporter (Pfcrt), multidrug resistance 1 (Pfmdr1), dihydrofolate reductase (Pfdhfr), dihydropteroate synthetase (Pfdhps), and ATPase 6 (PfATPase6) of P. falciparum. The results show that DNA microarray technology, combined with mPCR, is a promising and time-saving tool that supports conventional detection methods, allowing sensitive, accurate, simultaneous analysis of the SNPs associated with drug resistance in P. falciparum.
恶性疟原虫的耐药性对该寄生虫流行国家的公共卫生构成严重威胁。由于耐药性与寄生虫基因中的特定单核苷酸多态性（SNP）相关，分子标记正成为监测耐药性出现和传播的有用替代指标。在本研究中，开发了一种多重PCR（mPCR）和寡核苷酸微阵列方法，用于检测恶性疟原虫编码氯喹抗性转运蛋白（Pfcrt）、多药抗性1（Pfmdr1）、二氢叶酸还原酶（Pfdhfr）、二氢蝶酸合酶（Pfdhps）和ATP酶6（PfATPase6）的基因中的这些SNP。结果表明，DNA微阵列技术与mPCR相结合，是一种有前景且省时的工具，可支持传统检测方法，能够灵敏、准确、同时分析与恶性疟原虫耐药性相关的SNP。
